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Pakistan Journal of Biological Sciences

Year: 2009 | Volume: 12 | Issue: 5 | Page No.: 455-458
Research Article

The Study of Prevalence and Antimicrobial Susceptibility of Tracheal Bacterial Strains Isolated from Pediatric Patients

N. Jonaidi Jafari
Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
R. Ranjbar
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
M.T. Haghi-Ashtiani
Laboratory of Microbiology, Children`s Medical Center, School of Medicine, Medical Sciences/University of Tehran, Tehran, Iran
M. Abedini
Laboratory of Microbiology, Children`s Medical Center, School of Medicine, Medical Sciences/University of Tehran, Tehran, Iran
M. Izadi
Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
The aim of this study was to investigate the prevalence and antimicrobial susceptibility of bacterial strains isolated from tracheal specimens obtained from pediatric patients admitted to a major children hospital in Tehran, in 2007. Tracheal specimens were cultured on the appropriate bacteriological media. Bacterial isolates were identified by standard biochemical and serological tests. Antimicrobial susceptibility testing was performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Pseudomonas spp. was identified as the most prevalent bacterial isolate (32%) followed by Staphylococcus aureus (27.6%). Thirty strains (16%) were identified as Klebsiella spp., 18 (9.6%) as Enterobacter spp. and the rest belonged to coagolase negative Staphylococci, Streptococcus viridans, Acinetobacter spp., Escherichia coli and Neisseria spp. All Pseudomonas spp. were resistant to ampicllin, kanamycin and ceftizoxime. Staphylococcus and Klebsiella spp. showed high degree of resistance to 40% of examined antibiotics.
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References

  1. Bassetti, D., M. Bassetti and M. Mantero, 2000. Strategies for antibiotic selection in empirical therapy. Clin. Microbiol. Infect., 6: 98-100.
    PubMed
  2. Carroll, K. and L. Reimer, 1996. Microbiology and laboratory diagnosis of upper respiratory tract infections. Clin. Infect. Dis., 23: 442-448.
    PubMed
  3. Clinical and Laboratory Standards Institute, 2005. Guidelines by CLSI/NCCLS\-CLSI information supplement. Approved Standard M100-S15 Wayne, PA; 2000. http://wwwn.cdc.gov/cliac/pdf/Addenda/cliac0905/AddendumN.pdf.
  4. Creer, D.D., J.P. Dilworth, S.H. Gillespie, A.R. Johnston and S.L. Johnston et al., 2006. Aetiological role of viral and bacterial infections in acute adult lower respiratory tract infection (LRTI) in primary care. Thorax, 61: 75-79.
    PubMedDirect Link
  5. Dagan, R., K.P. Klugman, W.A. Craig and F. Baquero, 2001. Evidence to support the rationale that bacterial eradication in respiratory tract infection is an important aim of antimicrobial therapy. J. Antimicrob. Chemother., 47: 129-140.
    PubMed
  6. Weems, J.J. Jr., 2001. The many faces of Staphylococcus aureus infection. Recognizing and managing its life-threatening manifestations. Postgrad. Med., 110: 24-36.
    PubMed
  7. Kamat, S.R., S.S. Heera, P.V. Potdar, S.V. Shah, N.M. Bhambure and A.A. Mahasur, 1989. Bombay experience in intensive respiratory care over 6 years. J. Postgrad. Med., 39: 123-134.
    PubMedDirect Link
  8. Macfarlane, J.T., A. Colville, A. Guion and D.H. Rose, 1993. Prospective study of aetiology and outcome of adult lower-respiratory-tract infections in the community. Lancet, 341: 511-514.
    PubMedDirect Link
  9. Mizgerd, J.P. 2008. Acute lower respiratory tract infection. N. Engl. J. Med., 358: 716-727.
    PubMedDirect Link
  10. Navneeth, B.V and M.R. Sandhya Belwadi, 2002. Antibiotic resistance among gram-negative bacteria of lower respiratory tract secretion in hospitalised patients. Indian. J. Chest. Dis. Allied. Sci., 44: 173-176.
    PubMed
  11. Pezzlo, M., 1992. Aerobic Bacteriology. In: Clinical Microbiology Procedures Handbook, Isenberg, H.D. (Ed.). American Society for Microbiology, Washington, DC., ISBN: 1-55581-038-1, pp: 1.19.1-20.47.
  12. Pittet, D., 1994. Nosocomial pneumonia: Incidence, morbidity and mortality in the intubated-ventilated patients. Schweiz. Med. Woechensch., 124: 227-235.
    PubMed
  13. Reisner, S.B., G.L. Woods, R.P. Thomson, D.H. Larone, L.S. Garcia and R.Y. Shimuzu, 1999. Specimen Collection. In: Manual of Clinical Microbiology, Murray, P.R., E.J. Baron, M.A. Pfaller, F.C. Tenover and R.H. Yolken (Eds.). American Society for Microbiology, Washington, DC., ISBN: 1555811264, pp: 64-76.
  14. Varotto, F., G.D. Maria, R. Azzaro, P. Bellissima and R. Amato et al., 2001. An observational study on the epidemiology of respiratory tract bacterial pathogens and their susceptibility to four injectable beta-lactam antibiotics: Piperacillin, piperacillin/tazobactam, ceftazidime and ceftriaxone. J. Chemother., 13: 413-423.
    PubMedDirect Link
  15. Gonzalo de Liria, C.R., 2004. What is the importance of bacterial eradication in the treatment of respiratory tract infections? An. Pediatr. (Barc)., 60: 459-467.
    PubMedDirect Link
  16. Kumari, H.B.V., S. Nagarathna and A. Chandramuki, 2007. Antimicrobial resistance pattern among aerobic gram negative bacilli of lower respiratory tract specimens of intensive care unit patients in a neurocentre. Indian. J. Chest. Dis. Allied. Sci., 49: 19-22.
    PubMed
  17. Macfarlane, J., W. Holmes, P. Gard, R. Macfarlane and D. Rose et al., 2001. Prospective study of the incidence, aetiology and outcome of adult lower respiratory tract illness in the community. Thorax, 56: 109-114.
    CrossRefPubMedDirect Link
  18. Ranjbar, R., A. Aleo, G.M. Giammanco, A.M. Dionisi, N. Sadeghifard and C. Mammina, 2007. Genetic relatedness among isolates of Shigella sonnei carrying class 2 integrons in Tehran, Iran, 2002-2003. BMC. Infect. Dis., 7: 62-62.
    CrossRefPubMedDirect Link
  19. Ranjbar, R., C. Mammina, M.R. Pourshafie and M.M. Soltan Dallal, 2008. Characterization of endemic Shigella boydii strains isolated in Iran by serotyping, antimicrobial resistance, plasmid profile, ribotyping and pulsed-field gel electrophoresis. BMC Res. Notes, 1: 74-74.
    CrossRefPubMedDirect Link
  20. Ranjbar, R., M.M. Soltan Dallal, M. Talebi and M.R. Pourshafie, 2008. Increased isolation and characterization of Shigella sonnei obtained from hospitalized children in Tehran, Iran. J. Health Popul. Nutr., 26: 426-430.
    PubMedDirect Link
  21. Ranjbar, R., N. Sadeghifard, A. Ahmadi, M. Izadi and J. Zaeimi-Yazdi et al., 2007. Antimicrobial susceptibility and AP-PCR typing of Acinetobacter spp. strains. Iran. J. Publ. Health, 36: 50-56.
    Direct Link
  22. Shirazi, M.M., R. Ranjbar, F. Hemati and N. Sadeghifard, 2005. Bacterial infections in renal transplant recipients. Iran. J. Publ. Health, 34: 62-66.
    Direct Link
  23. Shirazi, M.M., R. Ranjbar, A. Ghasemi, S. Pakratigh, N. Sadeghifard and M.R. Pourmand, 2007. A survey of bacterial infections in bone marrow transplant recipients. Iran. J. Publ. Health, 36: 77-81.
    Direct Link

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