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Pakistan Journal of Biological Sciences

Year: 2005 | Volume: 8 | Issue: 9 | Page No.: 1221-1224
Research Article

Effect of UV-C Radiation on Drought Tolerance of Alfalfa (Medicago sativa) Callus

Ali Akbar Ehsanpour
Department of Biology, Isfahan University, Isfahan, Iran
Roya Razavizadeh
Department of Biology, Isfahan University, Isfahan, Iran
Water stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is located in a dry area, consequently, the study of plant responses to water stress is important. In this study UV-C radiation was used to induce physiological and genetic changes in alfalfa callus. In vitro grown calluses of M. sativa were exposed to UV-C for 0,15, 30 and 60 min and then, were transferred to MS medium containing 0, 2, 5, 10, 20 and 30% PEG. All explants were kept in the dark in the culture room. Calluses tolerate to osmotic stress were selected according to fresh weight. Results showed that radiation of UV-C for 60 min increased the osmotic tolerance and decreased the effect of drought stress.
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