Abstract:
Water stress and unfavorable climate decrease the growth and development of
plant globally. Medicago is a valuable plant as a source of food for
animal, foliage and for medicine. Since, Iran is located in a dry area,
consequently, the study of plant responses to water stress is important. In this
study UV-C radiation was used to induce physiological and genetic changes in
alfalfa callus. In vitro grown calluses of M. sativa were exposed
to UV-C for 0,15, 30 and 60 min and then, were transferred to MS medium
containing 0, 2, 5, 10, 20 and 30% PEG. All explants were kept in the dark in
the culture room. Calluses tolerate to osmotic stress were selected according to
fresh weight. Results showed that radiation of UV-C for 60 min increased the
osmotic tolerance and decreased the effect of drought stress.
Ali Akbar Ehsanpour and Roya Razavizadeh , 2005. Effect of UV-C Radiation on Drought Tolerance of Alfalfa (Medicago sativa)
Callus. Pakistan Journal of Biological Sciences, 8: 1221-1224.
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