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Journal of Biological Sciences

Year: 2013 | Volume: 13 | Issue: 4 | Page No.: 183-195
Research Article

Enzyme Activity and Histopathology of Rat Liver Treated with Crude Methanolic Extract of Pepperomia pellucida (L.) HBK

Kimberly S. Beltran-Benjamin
Department of Biology, College of Arts and Sciences, University of the Philippines Manila, Padre Faura St. Taft Avenue, Ermita, Manila, Philippines
Elisa L. Co
Department of Biology, College of Arts and Sciences, University of the Philippines Manila, Padre Faura St. Taft Avenue, Ermita, Manila, Philippines
Sally Andrea D. Gaspi
Department of Biology, College of Arts and Sciences, University of the Philippines Manila, Padre Faura St. Taft Avenue, Ermita, Manila, Philippines
Jessica Leika R. Matibag
Department of Biology, College of Arts and Sciences, University of the Philippines Manila, Padre Faura St. Taft Avenue, Ermita, Manila, Philippines
Glenn L. Sia Su
Department of Biology, College of Arts and Sciences, University of the Philippines Manila, Padre Faura St. Taft Avenue, Ermita, Manila, Philippines
The present study was designed to test the effect of the crude methanolic extract of Peperomia pellucida (L.) HBK on the antioxidant and redox enzymes. Twenty-five rats were divided into five groups that received different treatments. These rats were sacrificed and the liver was obtained to estimate the antioxidant superoxide dismutase (SOD) and catalase (CAT) and redox thioltransferase (TT) and thioredoxin reductase (TrxR) enzyme activities by spectrophotometry and to examine the physiological state of the liver by histopathological analysis. The crude methanolic extract of P. pellucida increased the activities of antioxidant enzymes (SOD and CAT) while redox enzyme (TT and TrxR) activities were greatly decreased. It was found out that 400 mg kg-1 b.wt. of P. pellucida extract was the most effective concentration and this finding was further supported by lesser incidence of fibrosis and necro-inflammation in the histopathological analysis of liver subjected to such dosage. Based on this result, it can be concluded that the crude methanolic extract of P. pellucida (400 mg kg-1 b.wt.) has a potent antioxidant activity because of its ability to elevate SOD and CAT and lower TT and TrxR activities under oxidative stress.
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How to cite this article

Kimberly S. Beltran-Benjamin, Elisa L. Co, Sally Andrea D. Gaspi, Jessica Leika R. Matibag and Glenn L. Sia Su, 2013. Enzyme Activity and Histopathology of Rat Liver Treated with Crude Methanolic Extract of Pepperomia pellucida (L.) HBK. Journal of Biological Sciences, 13: 183-195.

DOI: 10.3923/jbs.2013.183.195

URL: https://scialert.net/abstract/?doi=jbs.2013.183.195

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