Submit Manuscript

Journal of Biological Sciences

Year: 2008 | Volume: 8 | Issue: 7 | Page No.: 1216-1220
Research Article

In vitro Microrhizome Induction in Curcuma zedoaria (Christm.) Roscoe-A Conservation Prioritized Medicinal Plant

M. Anisuzzaman
Plant Biotechnology Laboratory, Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh
S.A. Sharmin
Plant Biotechnology Laboratory, Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh
S.C. Mondal
Plant Biotechnology Laboratory, Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh
R. Sultana
Plant Biotechnology Laboratory, Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh
M. Khalekuzzaman
Department of Genetic Engineering and Biotechnology, Rajshahi University, Rajshahi-6205, Bangladesh
I. Alam
Division of Applied Life Sciences (BK21), Gyeongsang National University, Jinju 660-701, Republic of Korea
M.F. Alam
Plant Biotechnology Laboratory, Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh
In vitro microrhizome was successfully produced in Curcuma zedoaria-a valuable but critically threatened medicinal plant. Ten to twelve weeks old in vitro multiplied shoots were cultured in MS medium supplemented with different level of BAP and NAA. Effect of different carbon sources has also been evaluated. Microrhizome formation started within 7-9 weeks of culture. The best result was obtained in 4.0 mg L-1 BAP along with 6% sucrose in terms of frequency and number of rhizome. Around 4 microrhizomes with an average weight of 2.5 g were harvested after 11-12 weeks. Microrhizomes were readily germinated in the growth regulator free MS medium. With the best of our knowledge this is the first report on in vitro microrhizome production in Curcuma zedoaria.
PDF Fulltext XML References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *

Article Trend

Total views 4252

References

  1. Alam, M.F., N. Ahsan, M. Anisuzzaman, S. Parvez, A.M. Swaraz and M.R. Khan, 2003. Effect of different kinds of carbon sources and concentrations on in vitro tuberization in potato (Solanum tuberosum L.) cv. diamant. Bangladesh J. Genet. Biotechnol., 4: 45-48.
  2. Balachandran, S.M., S.R. Bhat and K.P.S. Chandel, 1990. In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Rep., 8: 521-524.
    CrossRefDirect Link
  3. Bharalee, R., A. Das and M.C. Kalita, 2005. In vitro clonal propagation of Curcuma caesia Roxb and Curcuma zedoaria Rosc from rhizome bud explants. J. Plant Biochem. Biol., 14: 61-63.
    CrossRefDirect Link
  4. Chhetri, D.R., D. Basnet, P.F. Chiu, S. Kalikotay, G. Chhetri and S. Parajuli, 2005. Current status of ethnomedicinal plants in the Darjeeling Himalaya. Current Sci., 89: 264-268.
    Direct Link
  5. Chirangini, P. and G.J. Sharma, 2005. In vitro propagation and microrhizome induction in Zingiber cassumunar (Roxb.): An antioxidant rich medicinal plant. JFAE., 3: 139-142.
    Direct Link
  6. Egnin, M., L. Crawford and A. Sama, 2003. Development of an in vitro tuberization system for sweet potato micro-storage root formation. Abstract Congress In Vitro Cell. Dev. Biol., 39: 19-19.
  7. Haque, S. and A. Rashid, 1973. Characterization of the essential oil of Curcuma zedoaria. Bangladesh Pharm., 2: 19-23.
  8. Islam, M.A., K. Kloppstech and E. Esch, 2005. Population genetic diversity of Curcuma zedoaria (Christm.) Roscoe. A conservation prioritized medicinal plant in Bangladesh. Conserv. Genet., 6: 1027-1033.
    CrossRefDirect Link
  9. Liu, J.Y., 1996. A neoplasm inhibitor from traditional Chinese drug Curcuma zedoaria. Zhongcaoyao, 27: 572-572.
  10. Loc, N.H., D.T. Duc, T.H. Kwon and M.S. Yang, 2005. Micropropagation of zedoary (Curcum zedoaria Roscoe) a valuable medicinal plant. Plant Cell Tissue Org. Cult., 81: 119-122.
    CrossRef
  11. Maciel, N. and R.A. Criley, 2003. Morphology growth and flowering behavior of Curcuma zedoaria. Acta. Hortic. (ISHS), 624: 111-116.
    Direct Link
  12. Matsuda, H., K. Ninomiya, T. Morikawa and M. Yoshikawa, 1998. Inhibitory effect and action mechanism of sesquiterpebes for zedoaria rhizome on D-galactosamine/Lipopolysaccharide induced liver injury. Bioory. Med. Chem. Lett., 8: 339-344.
    Direct Link
  13. Minyi, C., 1992. Anticancer Medicinal Herbs. 1st Edn., Human Science and Technology Publishing House, Changsha.
  14. Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
    CrossRefDirect Link
  15. Nalawade, S. M., A.P. Sagare, C.Y. Lee, C.L. Kao and H.S. Tsay, 2003. Studies on tissue culture of Chinese medicinal plant resources in Taiwan and their sustainable utilization. Bot. Bull. Acad. Sin., 44: 79-98.
    Direct Link
  16. Nayak, S., 2000. In vitro multiplication and microrhizome induction in Curcuma aromatica salisb. J. Plant Growth Regul., 32: 41-47.
  17. Nayak, S., 2002. High frequency in vitro production of microrhizomes of Curcuma amada. Indian J. Exp. Biol., 40: 230-232.
    Direct Link
  18. Paisookasantivatana, Y., S. Kake, H. Seko, 2001. Genetic diversity of Curcuma alismaitfolia Gagenp. (Zingiberaceae) in Thailand as revealed by allozyme polymorphism. Genet. Res. Crop. Evol., 48: 459-465.
  19. Rout, G.R., S.K. Palai and P. Das, 2001. Onset of in vitro rhizogenesis response and peroxidase activity in Zingiber officinale (Zingiberaceae). Rev. Biol. Trop., 49: 965-971.
    Direct Link
  20. Salvi, N.D., L. George and S. Eapen, 2002. Micropropagation and field evaluation of micropropagated plants of turmeric. Plant Cell. Tissue Organ Cult., 68: 143-151.
    CrossRefDirect Link
  21. Sasaki, Y., H. Fushimi, H. Cao, S.Q. Cai and K. Komatsu, 2002. Sequcnce analysis of Chinese and Japaness Curcum drugs on the 185 rRNA gene and trnK gene the application of amplification refractory mutation system analysis for their authentication. Biol. Pharm. Bull., 25: 1593-1599.
    CrossRef
  22. Sharma, T.R. and B.M. Singh, 1995. In vitro microrhizome production in Zingiber officinale Rosc. Plant Cell Rep., 15: 274-277.
    CrossRefDirect Link
  23. Shibuya, H., M. Yoshihara, E. Kitano, M. Nagasawa and I. Kitagawa, 1986. Quantitative and qualitative analysis of essential oil constituents in various zedoaria rhizomes by GC/MS. Yakugaku Zasshi., 106: 212-216.
  24. Shirgurkar, M., C. John and R. Nadgauda, 2001. Factor affecting in vitro microrhizome production in turmeric. Plant Cell Tissue Org. Cult., 64: 5-11.
    CrossRefDirect Link
  25. Sunitibala, H., M. Damayanti and G.J. Sharma, 2001. In vitro propagation and rhizome formation in Curuma longa Linn. Cytobios., 105: 71-82.
    PubMedDirect Link
  26. Syu, W.J., C.C. Shen, M.J. Don, J.C. Ou, G.H. Lee and C.M. Sun, 1998. Cytotoxicity of curcuminoids and some novel compounds from Curcuma zedoaria. Nat. Prod., 61: 1531-1534.
    Direct Link
  27. Yoshioka, T., E. Fujii, Endo, M. Wada, Y. Tokunaga and N. Shibaet al., 1998. Antiinflammatory potency of dehydrocurdione a zedoary- derived sesquiterpene. Inflamm. Res., 47: 467-481.
    Direct Link
  28. Zheng, Y., Y. Liu, M. Ma and K. Xu, 2008. Increasing in vitro microrhizome production of ginger (Zingiber officinale Roscoe). Acta Physiol. Plantarum., 30: 513-519.
    CrossRefDirect Link

Keywords