
	International Journal of Poultry Science
	Year: 2010 \| Volume: 9 \| Issue: 8 \| Page No.: 813-818 DOI: 10.3923/ijps.2010.813.818

PCR Based Diagnosis of Eimeria tenella Infection in Broiler Chicken

Gautam Patra, M. Ayub Ali, Kh. Victoria Chanu, L. Jonathan, L.K. Joy, M. Prava, R. Ravindran, G. Das and L. Inaotombi Devi

Abstract: The present investigation was undertaken to evaluate histo-pathological, haemotological and biochemical changes in broiler chicken naturally infected with Eimeria tenella. A Polymerase Chain Reaction (PCR) based assay was also done for the detection, identification and differentiation of pathogenic species of Eimeria in poultry. Post mortem examination revealed petechial haemorrhages, oedema, necrosis and sloughing of caecal epithelium. Histopathological evidence showed leakage of blood, oedema and necrosis. Haemotological studies revealed that coccidial infection caused significant decrease in the value of haemoglobin and Packed Cell Volume (PCV). The value of Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin Concentration (MCHC) revealed that caecal coccidiosis resulted in macrocytic hypochromic anaemia. Biochemical serum analysis of coccidial infected chicken showed a significant increase in level of glucose, Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) while there was a significant decrease in the level of alkaline phosphatase (SAP) and cholesterol. In PCR using specific primers for E. tenella only the ITS 1 regions of ribosomal DNA (rDNA) could be amplified.

PDF References Citation Report Citation

RELATED ARTICLES:

•	Effect of Fish Oil on Performance, Coccidiosis Prevention and Serum Lipid Profile in Broiler

•	Transmission Electron Microscopy (TEM) Study of the Oocyst of Eimeria tenella

How to cite this article:

Gautam Patra, M. Ayub Ali, Kh. Victoria Chanu, L. Jonathan, L.K. Joy, M. Prava, R. Ravindran, G. Das and L. Inaotombi Devi, 2010. PCR Based Diagnosis of Eimeria tenella Infection in Broiler Chicken. International Journal of Poultry Science, 9: 813-818.

DOI: 10.3923/ijps.2010.813.818

URL: https://scialert.net/abstract/?doi=ijps.2010.813.818

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		Academic Journals Database
		AGRICOLA
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Genamics
		MIAR
		Stanford Libraries
		SUN Veterinary Medical Library

Science Alert