Submit Manuscript

Biotechnology

Year: 2015 | Volume: 14 | Issue: 1 | Page No.: 23-28
Research Article

Optimization of FPase Activity using Sorghum Straw Planted in Malaysia by Aspergillus terreus SUK-1 via Solid Substrate Fermentation

El Mubarak Musa Tibin Musa
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi, 43600, Selangor, Malaysia
Najeeb Kaid Nasser Al-Shorgani
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi, 43600, Selangor, Malaysia
Nawal Noureldaim Abuelhassan
School of Chemical Sciences and Food Technology, Faculty of Food Science and Technology, Universiti Kebangsaan Malaysia, Malaysia
Febri Doni
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi, 43600, Selangor, Malaysia
Wedad Hassan Abdelhaleem
Industrial Research and Consultancy Centre (IRCC), Khartoum, Sudan
Aidil Abdul Hamid
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi, 43600, Selangor, Malaysia
Mohd Sahaid Kalil
Department of Chemical and Process Engineering, Faculty of Engineering, Universiti Kebangsaan Malaysia, UKM, Bangi, 43600, Selangor, Malaysia
Wan Mohtar Wan Yusoff
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi, 43600, Selangor, Malaysia
The optimum conditions for cellulase activity measured as Filter paper activity (FPase) from sorghum straw by Aspergillus terreus SUK-1 using Response Surface Methodology (RSM) was based on Central Composite Design (CCD). The effect of incubation temperature, initial pH and moisture content on FPase activity was carried out in Solid Substrate Fermentation (SSF). The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. ANOVA analysis indicated that the model was significant (p<0.05) and that the effect of temperature and moisture content significantly effected on the FPase activity. The optimized conditions are; temperature 25°C, pH 4 and moisture content of 40%, while the predicted FPase activity is 0.35 U mL‾1. The model was validated by applying the optimized conditions and it was found that the FPase activity was 0.36 U mL‾1 which indicate the validity of the model.
PDF Fulltext XML References Citation

How to cite this article

El Mubarak Musa Tibin Musa, Najeeb Kaid Nasser Al-Shorgani, Nawal Noureldaim Abuelhassan, Febri Doni, Wedad Hassan Abdelhaleem, Aidil Abdul Hamid, Mohd Sahaid Kalil and Wan Mohtar Wan Yusoff, 2015. Optimization of FPase Activity using Sorghum Straw Planted in Malaysia by Aspergillus terreus SUK-1 via Solid Substrate Fermentation. Biotechnology, 14: 23-28.

DOI: 10.3923/biotech.2015.23.28

URL: https://scialert.net/abstract/?doi=biotech.2015.23.28

Leave a Comment

Your email address will not be published. Required fields are marked *

Article Trend

Total views 5118

References

  1. Reddy, B.V.S., S. Ramesh, P.S. Reddy, B. Ramaiah, P.M. Salimath and R. Kachapur, 2005. Sweet sorghum: A potential alternate raw material for bio-ethanol and bio-energy. J. SAT Agric. Res., 1: 1-8.
    Direct Link
  2. Zhang, C., G. Xie, S. Li, L. Ge and T. He, 2010. The productive potentials of sweet sorghum ethanol in China. Applied Energy, 87: 2360-2368.
    CrossRefDirect Link
  3. Vazquez, M., M. Oliva, S.J. Tellez-Luis and J.A. Ramirez, 2007. Hydrolysis of sorghum straw using phosphoric acid: Evaluation of furfural production. Bioresour. Technol., 98: 3053-3060.
    CrossRef
  4. Sonia, K.G., B.S. Chadha and H.S. Saini, 2005. Sorghum straw for xylanase hyper-production by Thermomyces lanuginosus (D2W3) under solid-state fermentation. Bioresour. Technol., 96: 1561-1569.
    CrossRef
  5. Al-Shorgani, N.K.N., E.M. Tibin, E. Ali, A.A. Hamid, W.M.W. Yusoff and M.S. Kalil, 2014. Biohydrogen production from agroindustrial wastes via Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). Clean Technol. Environ. Policy, 16: 11-21.
    CrossRef
  6. Mussatto, S.I. and I.C. Roberto, 2004. Alternatives for detoxification of diluted-acid lignocellulosic hydrolyzates for use in fermentative processes: A review. Bioresour. Technol., 93: 1-10.
    CrossRef
  7. Nystrom, J.M. and A.L. Allen, 1976. Pilot scale investigations and economics of cellulase production. Biotechnol. Bioeng. Symp., 6: 55-74.
    PubMed
  8. Rashid, S.A., D. Ibrahim and I.C. Omar, 2011. Utilization of palm kernel cake for the production of mannanase by an indigenous filamentous fungus, Aspergillus niger USM F4 under solid substrate fermentation. Internet J. Microbiol., 9: 1-19.
    Direct Link
  9. Yusoff, W.M.W., M.I. Massadeh, O. Omar and J. Kader, 2000. Sugar cane bagasse degradation by mixed culture of T. reesei and A. terreus in solid substrate fermentation. Pak. J. Biol. Sci., 3: 1758-1761.
    CrossRefDirect Link
  10. Damisa, D., J.B. Ameh and N.E.L. Egbe, 2011. Cellulase production by native Aspergillus niger obtained from soil environments. Ferment. Technol. Bioeng., 1: 62-70.
  11. Min, B.J., Y.S. Park, S.W. Kang, Y.S. Song and J.H. Lee et al., 2007. Statistical optimization of medium components for the production of xylanase by Aspergillus niger KK2 in submerged cultivation. Biotechnol. Bioprocess Eng., 12: 302-307.
    CrossRef
  12. Kansas State University, 1998. Grain sorghum production handbook. Kansas State University Agricultural Experiment Station and Cooperative Extension Service, Manhattan, KS., USA., May 1998. http://www.ksre.ksu.edu/bookstore/pubs/c687.pdf.
  13. Sternberg, D., P. Vuayakumar and E.T. Reese, 1977. β-Glucosidase: Microbial production and effect on enzymatic hydrolysis of cellulose. Can. J. Microbiol., 23: 139-147.
    CrossRefPubMed
  14. Hankin, L. and S.L. Anagnostakis, 1975. The use of solid media for detection of enzyme production by fungi. Mycologia, 67: 597-607.
    Direct Link
  15. Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31: 426-428.
    CrossRefDirect Link
  16. Latifian, M., Z. Hamidi-Esfahani and M. Barzegar, 2007. Evaluation of culture conditions for cellulase production by two Trichoderma reesei mutants under solid-state fermentation conditions. Bioresour. Technol., 98: 3634-3637.
    Direct Link
  17. Acharya, P.B., D.K. Acharya and H.A. Modi, 2008. Optimization for cellulase production by Aspergillus niger using saw dust as substrate. Afr. J. Biotechnol., 7: 4147-4152.
    Direct Link
  18. Ahmed, S., A. Bashir, H. Saleem, M. Saadia and A. Jamil, 2009. Production and purification of cellulose-degrading enzymes from a filamentous fungus Trichoderma harzianum. Pak. J. Bot., 41: 1411-1419.
    Direct Link
  19. Niranjane, A.P., P. Madhou and T.W. Stevenson, 2007. The effect of carbohydrate carbon sources on the production of cellulase by Phlebia gigantea. Enzyme Microbial. Technol., 40: 1464-1468.
    CrossRefDirect Link
  20. Karuppaiya, M., E. Sasikumar, T. Viruthagiri and V. Vijayagopal, 2009. Optimization of process conditions using Response Surface Methodology (RSM) for ethanol production from waste cashew apple juice by Zymomonas mobilis. Chem. Eng. Commun., 196: 1425-1435.
    CrossRef
  21. Oberoi, H.S., P.V. Vadlani, A. Nanjundaswamy, S. Bansal, S. Singh, S. Kaur and N. Babbar, 2011. Enhanced ethanol production from Kinnow mandarin (Citrus reticulata) waste via a statistically optimized simultaneous saccharification and fermentation process. Bioresour. Technol., 102: 1593-1601.
    CrossRef
  22. Ahmed, I., M.A. Zia, H. Muhammad and N. Iqbal, 2010. Bioprocessing of proximally analyzed wheat straw for enhanced cellulase production through process optimization with Trichoderma viride under SSF. Int. J. Biol. Sci., 6: 3164-3170.
  23. Wang, X.J., J.G. Bai and Y.X. Lian, 2006. Optimization of multienzyme production by two mixed strains in solid-state fermentation. Applied Microbiol. Biotechnol., 73: 533-540.
    CrossRefDirect Link
  24. Fang, X., Y. Shen, J. Zhao, X. Bao and Y. Qu, 2010. Status and prospect of lignocellulosic bioethanol production in China. Bioresour. Technol., 101: 4814-4819.
    CrossRef
  25. Narasimha, G., A. Sridevi, B. Viswanath, M.S. Chandra and R.B. Rajasekhar, 2006. Nutrient effects on production of cellulolytic enzymes by Aspergillus niger. Afr. J. Biotechnol., 5: 472-476.
    Direct Link
  26. Sohail, M., R. Siddiqi, A. Ahmad and S.A. Khan, 2009. Cellulase production from Aspergillus niger MS82: Effect of temperature and pH. N. Biotechnol., 25: 437-441.
    CrossRefPubMed
  27. Khurshid, S., S. Ali, H. Ashraf, M.A. Qadeer and M.I. Rajoka, 2001. Mutation of Aspergillus niger for hyperproduction of citric acid from black strap molasses. World J. Microbiol. Biotechnol., 17: 35-37.
    CrossRef
  28. Xiong, H., N. von Weymarn, M. Leisola and O. Turunen, 2004. Influence of pH on the production of xylanases by Trichoderma reesei Rut C-30. Process Biochem., 39: 731-736.
    CrossRef

Keywords