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Biotechnology
  Year: 2015 | Volume: 14 | Issue: 1 | Page No.: 23-28
DOI: 10.3923/biotech.2015.23.28
 
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Optimization of FPase Activity using Sorghum Straw Planted in Malaysia by Aspergillus terreus SUK-1 via Solid Substrate Fermentation

El Mubarak Musa Tibin Musa, Najeeb Kaid Nasser Al- Shorgani, Nawal Noureldaim Abuelhassan, Febri Doni, Wedad Hassan Abdelhaleem, Aidil Abdul Hamid, Mohd Sahaid Kalil and Wan Mohtar Wan Yusoff

Abstract:
The optimum conditions for cellulase activity measured as Filter paper activity (FPase) from sorghum straw by Aspergillus terreus SUK-1 using Response Surface Methodology (RSM) was based on Central Composite Design (CCD). The effect of incubation temperature, initial pH and moisture content on FPase activity was carried out in Solid Substrate Fermentation (SSF). The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. ANOVA analysis indicated that the model was significant (p<0.05) and that the effect of temperature and moisture content significantly effected on the FPase activity. The optimized conditions are; temperature 25°C, pH 4 and moisture content of 40%, while the predicted FPase activity is 0.35 U mL‾1. The model was validated by applying the optimized conditions and it was found that the FPase activity was 0.36 U mL‾1 which indicate the validity of the model.
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How to cite this article:

El Mubarak Musa Tibin Musa, Najeeb Kaid Nasser Al- Shorgani, Nawal Noureldaim Abuelhassan, Febri Doni, Wedad Hassan Abdelhaleem, Aidil Abdul Hamid, Mohd Sahaid Kalil and Wan Mohtar Wan Yusoff, 2015. Optimization of FPase Activity using Sorghum Straw Planted in Malaysia by Aspergillus terreus SUK-1 via Solid Substrate Fermentation. Biotechnology, 14: 23-28.

DOI: 10.3923/biotech.2015.23.28

URL: https://scialert.net/abstract/?doi=biotech.2015.23.28

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