Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
American Journal of Plant Physiology
  Year: 2010 | Volume: 5 | Issue: 6 | Page No.: 361-370
DOI: 10.3923/ajpp.2010.361.370
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Purification and Biochemical Characterization of Acid Phosphatase from Vigna aconitifolia

Mohammed A. Al-Omair

Abstract:
In present study, isolation and purification of Acid Phosphatase (AP) from the shoot of Vigna aconitifolia were conducted. The purification processes included the enzyme precipitation by ammonium sulphate and chromatographic adsorption by DEAE-cellulose and sephadex G200. This study showed a purification of AP up to 60 folds with specific activity of 280 U mg-1 protein. The optimal pH value was found to be 5.4. By studying the relationship between log V and pH, it was found that two amino acid residues namely cysteine and histidine are involved in the catalytic activity of AP. This indicate that Vigna aconitifolia AP is an –SH group dependent enzyme. The highest enzyme activity was recorded after 30 min of incubation in the reaction mixture. The optimal temperature for AP activity was 30°C. The activation energy was 0.44 kJ mol-1. After 50°C the Vigna aconitifolia AP activity was decreased continuously by prolongation of incubation period. When fructose-6-phosphate and sodium phytate were used instead of p-nitrophenyl phosphate (p-NPP), they expressed 44.3 and 67.1%, respectively of the enzyme activity with p-NPP as substrate. Km value for p-NPP was 133 mM and Vmax 27.8 nmol min-1.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Characterization of a Novel Surfactant and Organic Solvent Stable High-alkaline Protease from New Bacillus pseudofirmus SVB1
  •    Identification, Characterization and Partial Purification of Acid Phosphatase from Cotyledons of Psoralea corylifolia L.
How to cite this article:

Mohammed A. Al-Omair , 2010. Purification and Biochemical Characterization of Acid Phosphatase from Vigna aconitifolia. American Journal of Plant Physiology, 5: 361-370.

DOI: 10.3923/ajpp.2010.361.370

URL: https://scialert.net/abstract/?doi=ajpp.2010.361.370

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom