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Research Journal of Microbiology
  Year: 2011 | Volume: 6 | Issue: 11 | Page No.: 769-783
DOI: 10.17311/jm.2011.769.783
 
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Characterization of a Novel Surfactant and Organic Solvent Stable High-alkaline Protease from New Bacillus pseudofirmus SVB1

S. Sen, V. Venkata Dasu, K. Dutta and B. Mandal

Abstract:
Recently, alkaline proteases are of great importance due to their several industrial (detergent, tanning, textile and dairy industries, organic synthesis, peptide synthesis) and environmental applications. The objective of this study was to purify and characterize a novel surfactant and organic solvent stable high-alkaline protease from a new Bacillus pseudofirmus SVB1 (Shampa Venkata Bacillus isolate 1). The potential of the culture broth of a newly isolated Bacillus pseudofirmus SVB1 have evaluated in hydrolyzing natural proteins viz., egg albumin, blood, poultry feather and goat skin hair. An unusually large (85 kDa) serine alkaline protease was purified from the cell supernatant. High stability of the alkaline protease was found in the presence of surfactants, oxidizing agents, heavy metal ions and particularly organic solvents. The maximum activity was observed at an optimal pH of 10.0 and temperature of 40°C. The results obtained in this research study inferred that the purified alkaline protease was a novel surfactant and organic solvent tolerant enzyme with potential applications for various industrial processes.
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How to cite this article:

S. Sen, V. Venkata Dasu, K. Dutta and B. Mandal, 2011. Characterization of a Novel Surfactant and Organic Solvent Stable High-alkaline Protease from New Bacillus pseudofirmus SVB1. Research Journal of Microbiology, 6: 769-783.

DOI: 10.17311/jm.2011.769.783

URL: https://scialert.net/abstract/?doi=jm.2011.769.783

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