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Prevalence of Arcobacter spp. in Humans, Animals and Foods of Animal
Origin in India Based on Cultural Isolation, Antibiogram, PCR and Multiplex
PCR Detection |
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Hosakote Venkatappa Mohan,
Ramswaroop Singh Rathore,
Kuldeep Dhama,
Thadiyam Puram Ramees,
Anil Patya,
Prashanth Suresh Bagalko,
Mohd. Yaqoob Wani,
Kiran Narayan Bhilegaonkar and Ashok Kumar |
Abstract:
Arcobacter is an important emerging food and water borne pathogen having
worldwide public health concern. The present study reports the prevalence of
Arcobacter spp. in humans, animals and foods of animal origin based on
cultural isolation, antibiogram, Polymerase Chain Reaction (PCR) and multiplex
PCR detection. A total of 400 samples were collected as human diarrheal stool
(50), faecal swabs of poultry (50), pig (50), cattle (50) and foods of animal
origin [Raw milk (60), chicken meat (60), beef (40) and pork (40)]. The overall
prevalence rate of Arcobacter spp. was found to be 6.75% (27/400) by
cultural isolation with highest prevalence in pig faeces (12%), followed by
cattle faeces (10%), chicken meat (10%), poultry faeces (8%), beef (5%), pork
(5%), human diarrheal stools (2%) and milk (1.67%). PCR screening revealed prevalence
of Arcobacter spp. to be 7.75% (31/400) with highest in pig faeces (12%),
followed by cattle faeces (12%), chicken meat (11.67%), poultry (10%), beef
(7.5%), pork (5%), human stools (2.00%) and raw milk (1.67%). Multiplex PCR
assay enabled detection of A. butzleri (21/27) and A. skirrowii
(6/27). In vitro antibiotic sensitivity profile of 27 Arcobacter isolates
revealed most of these to be sensitive to azithromycin, gentamycin, nalidixic
acid, kanamycin, streptomycin, ciprofloxacin and tetracycline. Higher resistance
was observed for cephalothin, novobiocin and vancomycin with notable intermediately
resistance against erythromycin and chloramphenicol. The present study demonstrated
high prevalence of Arcobacter spp. in pig, cattle and poultry faecal
samples which may play important role in contamination of environment, water
and human food chain, thus could be of public health concerns. The PCR was found
to be more rapid, sensitive, specific and efficient than cultural methods for
detection of Arcobacter spp.
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How to cite this article:
Hosakote Venkatappa Mohan, Ramswaroop Singh Rathore, Kuldeep Dhama, Thadiyam Puram Ramees, Anil Patya, Prashanth Suresh Bagalko, Mohd. Yaqoob Wani, Kiran Narayan Bhilegaonkar and Ashok Kumar, 2014. Prevalence of Arcobacter spp. in Humans, Animals and Foods of Animal
Origin in India Based on Cultural Isolation, Antibiogram, PCR and Multiplex
PCR Detection. Asian Journal of Animal and Veterinary Advances, 9: 452-466.
DOI: 10.3923/ajava.2014.452.466
URL: https://scialert.net/abstract/?doi=ajava.2014.452.466
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