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Asian Journal of Animal and Veterinary Advances
  Year: 2014 | Volume: 9 | Issue: 9 | Page No.: 543-555
DOI: 10.3923/ajava.2014.543.555
 
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Molecular Characterization of Arcobacter Isolates Using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR)
P.S. Bagalakote, R.S. Rathore, T.P. Ramees, H.V. Mohan, M. Sumankumar, R.K. Agarwal, A. Kumar and K. Dhama

Abstract:
In modern years, Arcobacters are reflected as potential emerging food-borne zoonotic entero-pathogens. Arcobacter species displayed a wide variety of genetic diversity. The study was carried out to genotype and find molecular heterogeneity of Arcobacter spp. (Arcobacter butzleri, A. cryaerophilus and A. skirrowii), isolated from different sources from Bareilly region, Uttar Pradesh, India by using randomly amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR). RAPD-PCR was performed using genomic DNA of Arcobacter isolates (n = 56; 33 A. butzleri, 20 A. cryaerophilus, 3 A. skirrowii; recovered from chicken meat, pork, sheep faeces, goat faeces, poultry intestinal contents and human diarrhoeal stool samples) as template by employing two published primers. The RAPD profiling for primer 1 (HLWL85) yielded number of bands rangeing between 2-8 (500-3100 bp). Out of 56 isolates, 54 showed bands giving a typeability of 96.4%. These 54 typable strains were grouped to 35 types and giving discriminatory power of 0.9762. Primer 2 (OPA-11) yielded RAPD-PCR profiles comprising of 2-7 bands (210-2800 bp). Out of total 56 isolates, 54 were typable with a discriminatory power of 0.9336. This is the first report from India regarding RAPD profiling of Arcobacter spp. This study reveals epidemiological relationship of Arcobacter isolate from various sources and will help to design suitable prevention and control strategies for this important pathogen having public health significance.
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  •    Food-borne Pathogens of Animal Origin-Diagnosis, Prevention, Control and Their Zoonotic Significance: A Review
  •    Prevalence of Arcobacter spp. in Humans, Animals and Foods of Animal Origin in India Based on Cultural Isolation, Antibiogram, PCR and Multiplex PCR Detection
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How to cite this article:

P.S. Bagalakote, R.S. Rathore, T.P. Ramees, H.V. Mohan, M. Sumankumar, R.K. Agarwal, A. Kumar and K. Dhama, 2014. Molecular Characterization of Arcobacter Isolates Using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). Asian Journal of Animal and Veterinary Advances, 9: 543-555.

DOI: 10.3923/ajava.2014.543.555

URL: https://scialert.net/abstract/?doi=ajava.2014.543.555

 
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