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Asian Journal of Animal and Veterinary Advances

Year: 2012 | Volume: 7 | Issue: 7 | Page No.: 556-567
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Authors

Jun Fang

Meihu Ma

Yongguo Jin

Ning Qiu

Chan Wang

Guodong Ren

Xin Huang

Keywords

Research Article

Assessment of Salmonella enteritidis Viability in Egg White during Early Incubation Stages by Fluorescent Staining Method

Jun Fang, Meihu Ma, Yongguo Jin, Ning Qiu, Chan Wang, Guodong Ren and Xin Huang
Egg white was considered to play an important role in bacterial-resistance nature of developing embryo especially during early incubation. Previous works were not quite informative in the antimicrobial action of egg white. This study was performed to investigate the survival ability of Salmonella enteritidis in egg white during early incubation and to tentatively reveal underline anti-Salmonella enteritidis mechanisms of egg white using the combination of flow cytometric analysis and fluorescent strategy. Such properties were also tested at different incubation temperature and pH. The traditional plate count method was also included in this study and the results were in accordance with those of flow cytometric assessment. Following egg white treatment, Salmonella enteritidis was discriminated into three subpopulations by flow cytometric assessment: viable, dead and membrane-compromised cells. Furthermore, the results showed that with the extension of incubation time, the population giving green fluorescence increased steadily while red fluorescence emittance didn’t change a lot. The incubation temperature and pH were both significant factors in anti-Salmonella enteritidis properties of egg white. Besides, the main target for antimicrobial factors in egg white on Salmonella enteritidis cells was membrane integrity and the action mechanism of egg white was bacteriostatic rather than bactericidal effect during early incubation.
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