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Singapore Journal of Scientific Research
  Year: 2020 | Volume: 10 | Issue: 4 | Page No.: 438-444
DOI: 10.3923/sjsres.2020.438.444
 
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Antisnake Venom Effect of Diospyros mespiliformis Stem-bark Extract on Naja nigricollis Venom in Albino Rats

Ibrahim Sani , Abubakar Abdulhamid , Fatima Bello, Amina Sulaiman and Habiba Aminu

Abstract:
Background and Objective: Snakebite is one of the major health problems in Nigeria. It is frequently treated with parenteral administration of serum-based antivenins. Despite the success of the serum therapy, antivenins have some limitations particularly in relation to the neutralization of local tissue damage. Hence, this research was aimed at evaluating the antisnake venom potential of Diospyros mespiliformis stem-bark ethanol extract on Naja nigricollis (spitting cobra) venom in albino rats. Materials and Methods: The LD50 of the venom was determined using probit analysis and preliminary phytochemical screening was conducted using standard methods. For the antivenom activity evaluation of the plant extract, experimental animals were grouped into six; normal control, venom control, venom and extract (150 mg kg1 b.wt.), venom and extract (300 mg kg1 b.wt.), extract control, venom and conventional antivenin. Results: The LD50 of the venom was determined to be 0.380 mg kg1 b.wt. The result of the phytochemical screening revealed the presence of saponins, tannins, glycosides, terpenoids and alkaloids. The antisnake venom activity of the extract presented significant increased (p<0.05) in the Mean Survival Time (MST) of 6.72±0.64 and 16.99±1.24 h at extract dose of 150 and 300 mg kg1 b.wt., respectively when compared with the venom control group with MST of 0.82±0.03 h. Conclusion: Based on these findings, it was established that D. mespiliformis stem-bark extract has antisnake venom activity and can serve as lead in isolating the active principle that can be affordable and readily available.
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How to cite this article:

Ibrahim Sani, Abubakar Abdulhamid, Fatima Bello, Amina Sulaiman and Habiba Aminu, 2020. Antisnake Venom Effect of Diospyros mespiliformis Stem-bark Extract on Naja nigricollis Venom in Albino Rats. Singapore Journal of Scientific Research, 10: 438-444.

DOI: 10.3923/sjsres.2020.438.444

URL: https://scialert.net/abstract/?doi=sjsres.2020.438.444

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