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Research Journal of Phytochemistry
  Year: 2017 | Volume: 11 | Issue: 3 | Page No.: 150-169
DOI: 10.3923/rjphyto.2017.150.169
 
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Metabolite Profiling and Antioxidant Potency of Couroupita guianensis Aubl. Using LC-QTOF-MS Based Metabolomics

Mital Kaneria , Kalpna Rakholiya , Ruchi Jakasania, Rajesh Dave and Sumitra Chanda

Abstract:
Background and Objective: Couroupita guianensis Aubl. is found throughout India in plains, is native to South India and Malaysia, which is used in traditional medicine to treat colds, stomach aches, skin diseases, malaria and disinfect wounds. Optimization of the best extraction method for the extraction of antioxidant bioactive metabolite and identification of these metabolites by LC-QToF-MS technique has been carried out from this species. Present study was designed to find out the best method for the extraction of antioxidant compounds from Couroupita guianensis (C. guianensis) and identification of those metabolites. Materials and Methods: The extraction was done by six different methods: Decoction extraction, ethanolic maceration extraction, methanolic maceration extraction, cold percolation extraction, microwave assisted extraction and infusion extraction method. Antioxidant activity and total phenol content were determined in all different extracts of various extraction methods of C. guianensis leaf, stem and flowers. Antioxidant activity was tested by 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power. Metabolite profile was carried out by applying non-targeted LC-QToF-MS. Results: The results showed that the extracting solvent significantly altered the antioxidant property estimations of C. guianensis leaf, stem and flowers. The aqueous extract of leaf obtained by cold percolation method had maximum phenol and showed best DPPH free radical scavenging activity and ferric reducing antioxidant power, therefore, it was selected for the further characterization of active metabolites and total 39 compounds were identified. Conclusion: High correlations between phenolic compositions and antioxidant activities of various extracts were observed. Cold percolation extraction method proved to be the best extraction method for the extraction of antioxidant from C. guianensis. Total 39 compounds belongs to different groups were detected and identified from the most potent extract of C. guianensis.
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How to cite this article:

Mital Kaneria, Kalpna Rakholiya, Ruchi Jakasania, Rajesh Dave and Sumitra Chanda, 2017. Metabolite Profiling and Antioxidant Potency of Couroupita guianensis Aubl. Using LC-QTOF-MS Based Metabolomics. Research Journal of Phytochemistry, 11: 150-169.

DOI: 10.3923/rjphyto.2017.150.169

URL: https://scialert.net/abstract/?doi=rjphyto.2017.150.169

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