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Research Journal of Medicinal Plants
  Year: 2020 | Volume: 14 | Issue: 4 | Page No.: 156-166
DOI: 10.3923/rjmp.2020.156.166
 
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Carissa edulis and Gmelina arborea Reduce Cell Viability and Prevent Apoptosis in CaCO-2 and Hep-G2 Cells

Razack Osseni , Serge Moukha, Habib Ganfon, Théophile Mobio, Pascale Dozolme and Edmond E. Creppy

Abstract:
Background and Objective: Gmelina arborea and Carissa edulis are medicinal plants worldwide used and especially in Benin to manage various diseases. The present study aimed to evaluate the cytotoxic activity of extracts and fractions from the Gmelina arborea and Carissa edulis. Materials and Methods: Plants leaves were collected, dried and extracted. The crude extracts were then fractionated using ethyl acetate and butanol. Thin layer chromatography was applied to screen phytochemical components. CaCO-2 and Hep-G2 cells were cultured in DMEM medium for 24 h and exposed to the extracts with concentrations range from 0.05-7.5 mg mL1 for 48 h. Cell viability and cell growth were performed by using the neutral red uptake and MTT colorimetric assays. Furthermore, cytotoxicity mechanism was screened by quantifying Lactate Dehydrogenase (LDH) leakage in the extracellular medium. In addition, the caspase-3/7 activation and DNA fragmentation assays were performed for apoptosis cell death pathway. Results: Many secondary metabolites such as; anthracenes, naphthoquinones, alkaloids, saponins and flavonoids were identified in the both plant extracts. An important cell viability reduction and inhibition of the two cells growth were recorded. Necrosis was the major pathway of cell death almost at the highest concentration and on the other hand, neither caspases-3/7 activation nor DNA fragments showing ladder shapes were observed. Conclusion: Carissa edulis and Gmelina arborea contain phytochemical components which reduced significantly cell viability concentration-dependently in the two cell lines in which the cell death pathway may be by necrosis rather than apoptosis.
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How to cite this article:

Razack Osseni, Serge Moukha, Habib Ganfon, Théophile Mobio, Pascale Dozolme and Edmond E. Creppy, 2020. Carissa edulis and Gmelina arborea Reduce Cell Viability and Prevent Apoptosis in CaCO-2 and Hep-G2 Cells. Research Journal of Medicinal Plants, 14: 156-166.

DOI: 10.3923/rjmp.2020.156.166

URL: https://scialert.net/abstract/?doi=rjmp.2020.156.166

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