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Research Journal of Medicinal Plants

Year: 2016 | Volume: 10 | Issue: 1 | Page No.: 89-97
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Authors

Srividya Malkapuram

Srividya Malkapuram

Krishnan Venkataraman

Rucha Tongaonkar

Swamini Taran

Lakshmisireesha Kolla

Lakshman Rajagopalan

Keywords

Green Coffee Extract (GCE), an extract of Coffea arabica bean is a popular health supplement employed for anti-obesity and anti-diabetic effects. Here a hydroalcoholic extract of Green Coffee (GCE) was evaluated for its potential as a cardioprotective agent against Doxorubicin (Dox) induced cardiac insult in a H9C2 rat cardiomyocyte in vitro model system. The GCE was tested in an MTT viability assay using 1 μM Dox with and without GCE pretreatment at 50, 100 and 250 μg mL–1 concentrations. GCE was also tested for its free radical scavenging ability in a DPPH assay at 10 concentrations (500 μg mL–1 maximum concentration). To understand the mechanism of action of cardioprotection, mitochondrial membrane potential (Δψm) was compared between Dox treated cells with and without GCE pretreatment, using the JC-1 dye. Finally, the activation of caspase-3/7 was quantitated. Findings from the above experiments demonstrated that GCE rescued H9C2 cardiomyocytes from Dox induced loss of cell viability in a dose-dependent manner. While Dox treatment caused a clear decrease in the JC-1 ratio from 2 to 1.6 due to loss of Δψm, pre-treatment with GCE at 25, 50 and 100 μg mL–1 restored the JC-1 ratio to 1.6, 1.9 and 2.0, respectively. Dox treatment potently induced caspase 3/7 activity by 5 fold and pre-treatment with GCE at 100 and 500 μg mL–1 reduced this activation to 3.5 and 1.5 fold, respectively. This data clearly demonstrates that GCE is strongly cardioprotective against Dox induced cardiac insult and the mechanism of action is by blocking activation of intrinsic apoptotic pathway.
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How to cite this article

Srividya Malkapuram, Krishnan Venkataraman, Rucha Tongaonkar, Swamini Taran, Lakshmisireesha Kolla and Lakshman Rajagopalan, 2016. Green Coffee Extract Protects H9C2 Cardiomyocytes from Doxorubicin Induced Apoptosis. Research Journal of Medicinal Plants, 10: 89-97.

URL: https://scialert.net/abstract/?doi=rjmp.2016.89.97

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