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Research Journal of Medicinal Plants
  Year: 2014 | Volume: 8 | Issue: 4 | Page No.: 187-195
DOI: 10.3923/rjmp.2014.187.195
 
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Antimicrobial, Phytochemical and Toxicological Evaluation of Lawsonia inermis Extracts against Clinical Isolates of Pathogenic Bacteria

Mohammed Mehadi Hassan Chowdhury, Khadizatul Kubra and Sheikh Rashel Ahmed

Abstract:
The search of new antimicrobial agents is a current demand due to emerging resistance of pathogen against the currently available antimicrobial agents. The use of medicinal plants as natural substitute is the paramount area of research to overwhelm the drug resistance of infectious agents. In the present study, antimicrobial activity of Lawsonia inermis was investigated against clinical isolates of eight bacteria including five Gram negative (Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella pneumoniae, Shigella sonnei) and three Gram positive (Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis) using disc diffusion method. Four types of Lawsonia inermis extracts were prepared using methanol, chloroform, acetone and water as extraction solvents while DMSO (Dimethyl sulfoxide) and water as dissolution solvents. The rate and extent of bacterial killing was determined by Time-kill kinetic assay at 1xMIC of each bacterial isolate. The overall safety of Lawsonia inermis extracts was assessed in mice. The minimum value of MIC for different bacterial isolates ranged from 3-9.50 mg mL-1. At 1xMIC of each bacterial isolate, 3log10 decrease in CFU was recorded after 6 h of drug exposure and no growth was observed in all tested bacteria after 24 h of exposure except B. subtilis and S. epidermidis. No sign of toxidrome were observed during in vivo toxicity evaluation in mice at 300 mg kg-1 concentration. The present study provides the scientific rational for medicinal use of Lawsonia inermis. The use of Lawsonia inermis extracts is of great significance as substitute antimicrobial agent in therapeutics.
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How to cite this article:

Mohammed Mehadi Hassan Chowdhury, Khadizatul Kubra and Sheikh Rashel Ahmed, 2014. Antimicrobial, Phytochemical and Toxicological Evaluation of Lawsonia inermis Extracts against Clinical Isolates of Pathogenic Bacteria. Research Journal of Medicinal Plants, 8: 187-195.

DOI: 10.3923/rjmp.2014.187.195

URL: https://scialert.net/abstract/?doi=rjmp.2014.187.195

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