Submit Manuscript

Research Journal of Medicinal Plants

Year: 2013 | Volume: 7 | Issue: 2 | Page No.: 107-112
Research Article

Substitution of Combined Extracts of Gongronema latifolium and Nauclea latifolia with Insulin Requirement in the Management of Type I Diabetes

Grace Sylvester Effiong
Department of Clinical Pharmacy and Biopharmacy, Faculty of Pharmacy, University of Uyo, P.M.B. 1017, Uyo, Nigeria
Herbert O.C. Mbagwu
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Nigeria
Imoh E. Udoh
Department of Clinical Pharmacy and Biopharmacy, Faculty of Pharmacy, University of Uyo, P.M.B. 1017, Uyo, Nigeria
Nsikan M. Udo
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Nigeria
Item J. Atangwho
Department of Biochemistry, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Nigeria
Patrick E. Ebong
Department of Biochemistry, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Nigeria
Ekpo N. Asuquo
Department of Biochemistry, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Nigeria
Lucy A. Wilson
Department of Chemistry, Akwa Ibom State College of Arts and Science, Nung Ukim Ikono, Nigeria
Polyherbal therapy, sometimes called polyherbalism-a combination of herbs or phytochemicals from more than one source, has today become accepted as an effective therapeutic approach in sourcing medicament for degenerative ailments. The present study, investigated the effect of combined extracts from Gongronema latifolium (GL) and Nauclea latifolia (NL) on some hormonal indices of normal and diabetic rats. Type 1 diabetes was induced with streptozotocin (50 mg kg-1 b.w.) and the hormonal parameters were measured using standard methods. Measured c-peptide levels showed significant increase in diabetic rats treated with the combined extracts and only GL relative to non-diabetic control. Whereas there were significant increase (p<0.05) in serum triiodothyronine (T3) and tetraiodothyronine (T4) concentrations of diabetic control when compared to normal control, administration of both combined extracts and insulin caused significant reduction (p<0.05) in T3 and T4 relative to the diabetic control, although, this observation differed in the non diabetic test animals. Measured serum insulin level was significantly reduced (p<0.05) in untreated diabetic rats relative to normal control, administration of only GL and its combination with NL extracts also reduced the level in a manner similar to insulin administration. On the contrary, normal rats that received similar treatments showed significant increase (p<0.05) in serum insulin level. Therefore, combined extracts of Gongronema latifolium and Nauclea latifolia may mimic insulin in its antihyperglycemic action at least in rats.
PDF Fulltext XML References Citation

How to cite this article

Grace Sylvester Effiong, Herbert O.C. Mbagwu, Imoh E. Udoh, Nsikan M. Udo, Item J. Atangwho, Patrick E. Ebong, Ekpo N. Asuquo and Lucy A. Wilson, 2013. Substitution of Combined Extracts of Gongronema latifolium and Nauclea latifolia with Insulin Requirement in the Management of Type I Diabetes. Research Journal of Medicinal Plants, 7: 107-112.

URL: https://scialert.net/abstract/?doi=rjmp.2013.107.112

Leave a Comment

Your email address will not be published. Required fields are marked *

Article Trend

Total views 2981

References

  1. Okoli, R.I., O. Aigbe, J.O. Ohaju-Obodo and J.K. Mensah, 2007. Medicinal herbs used for managing some common ailments among Esan people of Edo State, Nigeria. Pak. J. Nutr., 6: 490-496.
    CrossRefDirect Link
  2. Focho, D.A., W.T. Ndam and B.A. Fonge, 2009. Medicinal plants of Aguamb-Bamumbu in the Lebialem highlands, South West Province of Cameroon. Afr. J. Pharmacy Pharmacol., 3: 001-013.
  3. Nwanjo, H.U., M.C. Okafor and G.O. Oze, 2006. Anti-lipid peroxidative activity of Gongronema latifolium in streptozotocin-induced rats. Niger. J. Physiol. Sci., 21: 61-65.
    CrossRefPubMedDirect Link
  4. Ugochukwu, N.H. and N.E. Babady, 2003. Antihyperglycemic effect of aqueous and ethanolic extracts of Gongronema latifolium leaves on glucose and glycogen metabolism in livers of normal and streptozotocin-induced diabetic rats. Life Sci., 73: 1925-1938.
    CrossRefDirect Link
  5. Ugochukwu, N.H., N.E. Babady, M. Cobourne and S.R. Gasset, 2003. The effect of Gongronema latifolium extracts on serum lipid profile and oxidative stress in hepatocytes of diabetic rats. J. Biosci., 28: 1-5.
    CrossRefPubMedDirect Link
  6. Nwanjo, H.U., 2005. Effect of aqueous extract of Gongronema latifolium leaf on blood glucose level in rats. Alvana J. Sci., 1: 84-89.
  7. Nwanjo, H.U. and E.O. Alumanah, 2005. Effect of aqueous extract of Gongronema latifolium leaf on some indices of liver function in rats. Global J. Med. Sci., 4: 29-32.
  8. Gidado, A., D.A. Ameh and S.E. Atawodi, 2005. Effects of Nauclea latifolia leaves aqueous extracts on blood glucose levels of normal and alloxan-induced diabetic rats. Afr. J. Biotechnol., 4: 91-93.
    CrossRefDirect Link
  9. Gidado, A., Ameh, D.A., S.E. Atawodi and S. Ibrahim, 2008. Hypoglycaemic activity of Nauclea latifolia Sm. (Rubiaceae) in experimental animals. Afr. J. Tradit. Complementary Altern. Med., 5: 201-208.
    CrossRefPubMedDirect Link
  10. Atangwho, I.J., P.E. Ebong, G.E. Egbung and A.U. Obi, 2010. Extract of Vernonia amygdalina del. (African bitter leaf) can reverse pancreatic cellular lesion after alloxan damage in the rat. Aust. J. Basic Applied Sci., 4: 711-716.
  11. Ghoraishian, S.M., 2006. The effect of hazel-leaf decoction on blood glucose reduction in the diabetic rats. World J. Med. Sci., 1: 144-146.
    Direct Link
  12. Rao, P.V. and M.D. Naidu, 2010. Anti diabetic effect of Rhinacanthusnasutus leaf extract in streptozotocin induced diabetic rats. Libyan Agric. Res. Center J. Int., 1: 310-312.
    Direct Link
  13. Atangwho, I.J., 2008. Biochemical impact of combined administration of extracts of Vernonia amygdalina and Azadirachta indica leaves on STZ diabetic rat models. Ph.D. Thesis, University of Calabar, Calabar.
  14. Eastham, R.O., 1985. Biochemical Values in Clinical Medicine. 7th Edn., Wright Bristol, USA.
  15. Gharib, H., R.J. Ryan, W.E. Mayberry and T. Hockett, 1971. Radioimmunoassay for triiodothyronine (T3): Affinity and specificity of antibody for T3. J. Clin. Endocrinol., 33: 509-516.
    CrossRefDirect Link
  16. Starr, J.I., M.E. Mako, D. John and A.H. Rubenstein, 1978. Measurement of serum proinsulin-like material: cross-reactivity of porcine and human proinsulin in the insulin radioimmunoassay. J. Lab. Clin. Med., 91: 691-692.
    PubMedDirect Link
  17. Champe, P.C., R.A. Harvey and D.R. Ferrier, 2005. Biochemistry. In: Lippincott's Illustrated Reviews, Harvey, R.A. and P.C. Champe (Eds.). Lippincott Williams and Wilkins, USA.
  18. Karam, J.H. and P.H. Forsham, 1994. Pancreatic Hormones and Diabetic Mellitus. In: Basic and Clinical Endocrinology, Greenspan, F.S. and J.D. Baxter (Eds.). Appleton and Lange, East Norwalk, Conn, USA .
  19. Brandenburg, D., 2008. History and diagnostic significance of C-peptide. Exp. Diabetes Res., Vol. 2008.
    CrossRefDirect Link
  20. Mayes, P.A., 2000. Gluconeogenesis and Control of the Blood Glucose. In: Harper's Biochemistry, Murray, R.K., D.K. Granner, P.A. Mayes and V.W. Rodwell (Eds.). McGraw-Hill, New York, USA.

Keywords