Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Research Journal of Medicinal Plants
  Year: 2012 | Volume: 6 | Issue: 6 | Page No.: 406-415
DOI: 10.3923/rjmp.2012.406.415
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

TNF-α Production Inhibitory Activity, Phenolic, Flavonoid and Tannin Contents of Selected Indonesian Medicinal Plants

I. Batubara, S. Kotsuka, K. Yamauchi, H. Kuspradini, T. Mitsunaga and L.K. Darusman

Abstract:
In this study 28 Indonesian medicinal plants species has been screened as potential Tumor Necrosis Factor (TNF)-α inhibitor. Plant materials were extracted with methanol. The activity test was performed on human monocyte-derived THP-1 (ATCC No. TIB 202) which was stimulated by Lipopolyssacharide (LPS). The TNF-α production and cell viability were determined by commercial ELISA kit and WST-1, respectively. All extracts were determined for their phenolic, flavonoid and tannin contents by spectrophotometry and chromatography methods. From 40 plant materials, 5 plant materials had cell viability lower than 70% at 10 ppm concentration and about 8 plant materials had good cell viability higher than 90% at 10 ppm concentration. Among 8 plant materials extract which had good cell viability, only Avicenia sp. stem and Goniothalamus macrophyllus leaf methanol extracts had high TNF-α inhibitory activity. The total phenolic, flavonoid and tannin contents were varied depending on the sample. No correlation was found between TNF-α inhibitory activity with the total phenolic content, flavonoid content and tannin content. In conclusion, among Indonesian medicinal plants, the highest potential as TNF-α inhibitors were Avicenia sp. stem and G. macrophyllus leaves.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Phytosome: A Novel Approach Towards Functional Cosmetics
  •    Potency of Indonesian Medicinal Plants as Tyrosinase Inhibitor and Antioxidant Agent
  •    Indonesian Kepel Fruit (Stelechocarpus burahol) as Oral Deodorant
  •    Anti-inflammatory and Antipyretic Activities of Indian Medicinal Plant Cassia fistula Linn. (Golden Shower) in Wistar Albino Rats
How to cite this article:

I. Batubara, S. Kotsuka, K. Yamauchi, H. Kuspradini, T. Mitsunaga and L.K. Darusman, 2012. TNF-α Production Inhibitory Activity, Phenolic, Flavonoid and Tannin Contents of Selected Indonesian Medicinal Plants. Research Journal of Medicinal Plants, 6: 406-415.

DOI: 10.3923/rjmp.2012.406.415

URL: https://scialert.net/abstract/?doi=rjmp.2012.406.415

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 

Curve Bottom