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Research Journal of Medicinal Plants
  Year: 2008 | Volume: 2 | Issue: 1 | Page No.: 1-9
DOI: 10.3923/rjmp.2008.1.9
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Micropropagation, Isolation and Characterization of Berberine from Leaves of Naravelia zeylanica (L.) DC.

H. Raja Naika and V. Krishna

An in vitro regeneration protocol was standardized using leaf explants of Naravelia zeylanica (Ranunculaceae) a rare medicinal plant of the Western Ghats, India. The adventitious shoot buds were organized directly from mid veins and margin of the excised leaf explants cultured on MS-medium fortified with a range of 2.0 to 3.0 mg L-1 BAP and 0.3 to 0.7 mg L-1 IBA. The frequency of shoot bud organogenesis was the highest (14.9±0.27 shoots per explants) at the concentration of 2.5 mg L-1 BAP and 0.5 mg L-1 IBA. The excised micro shoots were pretreated in 0.5 mg L-1 IBA and transferred to MS half strength semisolid medium induced root initials from the cut ends. A mean of 13.5±0.30 root intact plantlets was recovered per explants showed morphological similarity with the in vivo plants. Two hundred and fifty gram of the powdered leaves from in vivo plants of N. zeylanica was subjected to Soxhlet extraction using methanol. Ten gram of each of the extracts were used for total alkaloid isolation and the yield was 270 mg. An alkaloid berberine was isolated by preparative TLC method using the solvent system methanol, water and ammonium hydroxide in the ratio 8: 1: 1 v/v. The characterization of the constituent was confirmed by IR, 1HNMR and Mass spectral studies.
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How to cite this article:

H. Raja Naika and V. Krishna, 2008. Micropropagation, Isolation and Characterization of Berberine from Leaves of Naravelia zeylanica (L.) DC.. Research Journal of Medicinal Plants, 2: 1-9.

DOI: 10.3923/rjmp.2008.1.9






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