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Research Journal of Botany

Year: 2015 | Volume: 10 | Issue: 1 | Page No.: 30-36
Research Article

Studies on Phytochemical Screening and in vitro Antioxidant Activity of Ethyl Acetate Leaf Extract of Justicia gendarussa Burm. F.

S. Nirmalraj
P.G Research Department of Botany, Government Arts College, Nandanam, Chennai, 600035, India
K. Perinbam
P.G Research Department of Botany, Government Arts College, Nandanam, Chennai, 600035, India
Plant antioxidants have gained significant interest in recent years for their role in preventing the auto corrosion of fats and oils. The present study was aimed to screen the phytochemical constituents such as alkaloids, anthraquinons, cumarins, flavonoids, glycosides, phenols, saponins, steroids and terpenoids from the ethyl acetate leaf extract of Justicia gendarussa and also to assess the antioxidant potential of same extract. Result of present investigation clearly revealed that total phenolic content was 34.10±0.79 mg/100 mg GAE and total flavonoid was found to be 15.01±0.30 mg/100 mg QE. In vitro antioxidant activity of J. gendarussa leaf by DPPH assay showed 74.20±0.44%, FRAP assay showed 72.15±0.44% and nitric acid scavenging assay revealed 65.99±1.93%, remarkably high phenolic content and antioxidant activities were found in the ethyl acetate extract. Further studies, to isolate and identification of bioactive compounds in food and pharmaceutical industries.
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S. Nirmalraj and K. Perinbam, 2015. Studies on Phytochemical Screening and in vitro Antioxidant Activity of Ethyl Acetate Leaf Extract of Justicia gendarussa Burm. F. Research Journal of Botany, 10: 30-36.

URL: https://scialert.net/abstract/?doi=rjb.2015.30.36

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References

  1. Yildirim, A., M. Oktay and V. Bilaloglu, 2001. The antioxidant activity of the leaves of Cydonia vulgaris. Turk. J. Med. Sci., 31: 23-27.
    Direct Link
  2. Davies, K.J.A., 1995. Oxidative stress: The paradox of aerobic life. Biochem. Soc. Symp., 61: 1-31.
    CrossRefPubMedDirect Link
  3. Robinson, E.E., S.R.J. Maxwell and G.H.G. Thorpe, 1997. An investigation of the antioxidant activity of black tea using enhanced chemiluminescence. Free Radical Res., 26: 291-302.
    CrossRefDirect Link
  4. Buyukokuroglu, M.E., I. Gulcin, M. Oktay and O.I. Kufrevioglu, 2001. In vitro antioxidant properties of dantrolene sodium. Pharmacol. Res., 44: 491-494.
    CrossRefPubMedDirect Link
  5. Farber, J.L., 1994. Mechanisms of cell injury by activated oxygen species. Environ. Health Perspect., 102: 17-24.
    PubMed
  6. Fang, Y.Z., S. Yang and G. Wu, 2002. Free radicals, antioxidants, and nutrition. Nutrition, 18: 872-879.
    CrossRefDirect Link
  7. Lee, J., N. Koo and D.B. Min, 2004. Reactive oxygen species, aging and antioxidative nutraceuticals. Compreh. Rev. Food Sci., 3: 21-33.
    CrossRefDirect Link
  8. Cuzzocrea, S., D.P. Riley, A.P. Caputi and D. Salvemini, 2001. Antioxidant therapy: A new pharmacological approach in shock, inflammation and ischemia/reperfusion injury. Pharmacol. Rev., 53: 135-159.
    PubMedDirect Link
  9. Periyanayagam, K., B. Umamaheswari, L. Suseela, M. Padmini and M. Ismail, 2009. Evaluation of antiangiogenic effect of the leaves of Justicia gendarussa (Burm. f ) (Acanthaceae) by chrio allontoic membrane method. Am. J. Infect. Dis., 5: 180-182.
    CrossRefDirect Link
  10. Krishna, K.L., T.A. Mehta and J.A. Patel, 2010. In-vitro hepatoprotective activity of Justicia gendarussa stem on isolated rat hepatocytes. Pharmacologyonline, 2: 9-13.
    Direct Link
  11. Trease, G.E. and W.C. Evans, 1989. Pharmacognosy. 11th Edn., Macmillan Publishers, London, UK.
  12. Harborne, J.B., 1998. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd Edn., Springer Science & Business Media, Berlin, Heidelberg, Germany, ISBN-13: 9780412572708, Pages: 302.
    Direct Link
  13. Shetty, K., O.F. Curtis, R.E. Levin, R. Witkowsky and W. Ang, 1995. Prevention of verification associated with in vitro shoot culture of oregano (Origanum vulgare) by Pseudomonas spp. J. Plant Physiol., 147: 447-451.
    CrossRefDirect Link
  14. Garrett, A.R., B.K. Murray, R.A. Robison and K.L. O'Neill, 2009. Measuring Antioxidant Capacity using the ORAC and TOSC Assays. In: Advanced Protocols in Oxidative Stress II, Armstrong, D. (Ed.). Springer, New York, USA., pp: 251-262.
    CrossRef
  15. Garrat, D.C., 1964. The Quantitative Analysis of Drugs. Vol. 3, Chapman and Hall, Japan, ISBN: 8123907540, pp: 456-458.
  16. Bidchol, A.M., A. Wilfred, P. Abhijna and R. Harish, 2011. Free radical scavenging activity of aqueous and ethanolic extract of Brassica oleracea L. var. italica. Food Bioprocess Technol., 4: 1137-1143.
    CrossRefDirect Link
  17. Miliauskas, G., P.R. Venskutonis and T.A. van Beek, 2004. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem., 85: 231-237.
    CrossRefDirect Link
  18. Chanda, S.V. and K.V. Nagani, 2010. Antioxidant capacity of Manilkara zapota L. leaves extracts evaluated by four in vitro methods. Nat. Sci., 8: 260-266.
    Direct Link
  19. Velioglu, Y.S., G. Mazza, L. Gao and B.D. Oomah, 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food Chem., 46: 4113-4117.
    CrossRefDirect Link
  20. Javanmardi, J., C. Stushnoff, E. Locke and J.M. Vivanco, 2003. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem., 83: 547-550.
    CrossRefDirect Link
  21. Kähkönen, M.P., A.I. Hopia, H.J. Vuorela, J.P. Rauha, K. Pihlaja, T.S. Kujala and M. Heinonen, 1999. Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem., 47: 3954-3962.
    CrossRefPubMedDirect Link
  22. Oyaizu, M., 1986. Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. Diet., 44: 307-315.
    CrossRefDirect Link
  23. Benzie, I.F.F. and J.J. Strain, 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal. Biochem., 239: 70-76.
    CrossRefPubMedDirect Link

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