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Research Journal of Botany
  Year: 2006 | Volume: 1 | Issue: 1 | Page No.: 6-18
DOI: 10.3923/rjb.2006.6.18
 
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Regulation of Glutathione under Abiotic Stress in Mutant and Wild Type Arabidopsis thaliana

Abdelfattah Badr, Salwa Badr , Safaa Radwan and David Oliver

Abstract:
The regulation of the GSH metabolic genes under stress was investigated by studying the mRNA expression pattern for the GGT genes in Arabidopsis plants grown in liquid culture system. Plants were exposed for 6 h to the following treatments: H2O2 (5 mM), salicylic acid S.A. (100 μM), jasmonic acid J.A. (100 μM), 2,4-D (100 μM) and CdCI2 (100 μM). GGT1 messages were found to be upregulated under H2O2, S.A, 2,4-D and CdC12. Meanwhile, GGT3/mRNAs were induced only under jasmonic acid with no further induction under the other stress treatments. In contrast, the GGT2/mRNAs were not greatly affected by different stress treatments, however, it showed some minor induction under S.A. and 2,4-D treatments. The investigation of the involvement of GGT1 gene in stress tolerance by following the behavior of ggtl mutants along with their characteristic phenotype under different stress conditions revealed that, the highly stressful conditions of light intensity (200 and 400 μmol m-2s-1) or temperature treatments (14 and 25°C) enhanced the GGT1 in the mutant phenotype above or below the optimal degree, rather than the combinations between them. This enhancement is recognized through the wider distribution of the chlorotic regions on the ggtl mutant leaves, the reduction in rosette diameter and the early blot pattern, rather than the reduction in the total weight of dry seeds/plant. In addition, the mutants responded more significantly to the high concentrations of 2,4-D (100, 200 and 400 μM) by enhancing the mutant phenotype.
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How to cite this article:

Abdelfattah Badr, Salwa Badr , Safaa Radwan and David Oliver , 2006. Regulation of Glutathione under Abiotic Stress in Mutant and Wild Type Arabidopsis thaliana. Research Journal of Botany, 1: 6-18.

DOI: 10.3923/rjb.2006.6.18

URL: https://scialert.net/abstract/?doi=rjb.2006.6.18

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