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Plant Pathology Journal
  Year: 2015 | Volume: 14 | Issue: 3 | Page No.: 97-112
DOI: 10.3923/ppj.2015.97.112
ATMPK4 and ATMPK6 Transcript and Protein Profiling in Arabidopsis thaliana Plants Challenged with Zeatin and Alternaria brassicae
D. Jeena, M. Baunthiyal, D. Pandey and A.K. Gupta

Abstract:
Mitogen-Activated Protein (MAP) kinases play very important role in signal transduction during pathogen attack. We attempted to study the response of MAPK4 and MAPK6 under three different conditions i.e., with pathogen (Alternaria brassicae) only, with zeatin only and with both pathogen and zeatin. The study was performed on Arabidopsis thaliana (A. thaliana) plants. The objective was to identify the relationship between zeatin and disease resistance. To our surprise, we found an increased disease incidence in presence of pathogen at middle stage along with zeatin and also observed increased expression of MAPK4 under similar condition. MAPK6 expressions are already known to be increased during the disease incidence and in our case it has increased expression during the application of both pathogen as well as zeatin. Two-dimensional (2D) analysis of these samples revealed that the upregulated proteins were mostly in the range of 35-50 kDa range which is similar to the range of where all the MAP kinases.
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How to cite this article:

D. Jeena, M. Baunthiyal, D. Pandey and A.K. Gupta, 2015. ATMPK4 and ATMPK6 Transcript and Protein Profiling in Arabidopsis thaliana Plants Challenged with Zeatin and Alternaria brassicae. Plant Pathology Journal, 14: 97-112.

DOI: 10.3923/ppj.2015.97.112

URL: https://scialert.net/abstract/?doi=ppj.2015.97.112

 
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