Diversity in Indian Isolates of Alternaria brassicicola (Schwein) Wiltshire Causing Black Leaf Spot Disease in Cauliflower


	Plant Pathology Journal
	Year: 2014 \| Volume: 13 \| Issue: 4 \| Page No.: 232-245 DOI: 10.3923/ppj.2014.232.245

Diversity in Indian Isolates of Alternaria brassicicola (Schwein) Wiltshire Causing Black Leaf Spot Disease in Cauliflower

Swati Deep, Pratibha Sharma, Niranjan Behera and Pallem Chowdappa

Abstract: Morphological, cultural, pathogenic and genetic variability in thirty two Alternaria brassicicola (Schwein) Wiltshire isolates infecting cauliflower (Brassicae oleracea var botrytis) from different parts of India were studied here. Dark olivaceous black fungal colonies were observed with small, obpyrifom, septate, brown colored spores forming in chain having no beak. A significant (p<0.05) morphological variability was found within the isolates in respect to conidia length, width and number of septa whereas less cultural variability was seen with respect to colony colour and growth. High sporulation intensity was found in most of the isolates. RAPD (42) and ISSR (12) primers generated a total of 337 amplicons out of which 16 were monomorphic, indicating presence of high level of genetic diversity within the pathogenic A. brassicicola isolates. No major clusters were formed, rather isolates of mixed geographical regions of collection are found to be interlinked with each other. The internal transcribed spacer region of all the thirty two isolates showed 100% similarity with A. brassicicola accessions from NCBI.

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How to cite this article:

Swati Deep, Pratibha Sharma, Niranjan Behera and Pallem Chowdappa, 2014. Diversity in Indian Isolates of Alternaria brassicicola (Schwein) Wiltshire Causing Black Leaf Spot Disease in Cauliflower. Plant Pathology Journal, 13: 232-245.

DOI: 10.3923/ppj.2014.232.245

URL: https://scialert.net/abstract/?doi=ppj.2014.232.245

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