Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Plant Pathology Journal
  Year: 2014 | Volume: 13 | Issue: 3 | Page No.: 160-166
DOI: 10.3923/ppj.2014.160.166
Management of Cucumber Wilt Disease by Bacillus subtilis B006 Through Suppression of Fusarium oxysporum f. sp. cucumerinum in Rhizosphere
Qi-Yao Yang, Ke Jia, Wen-Yi Geng, Rong-Jun Guo and Shi-Dong Li

Abstract:
Bacillus subtilis B006 has been attempted in this study to control wilt disease of cucumber. In order to clear its suppressing mechanism in rhizosphere, population of Fusarium oxysporum f. sp. cucumerinum J. H. Owen (Foc) and B006 in cucumber rhizosphere were determined using real-time PCR. Real-time PCR program of B. subtilis B006 in soil was established for first time with the limit as low as 78.0 CFU g-1. Real-time PCR assays showed that combined use of B006 in substrate, drenching during transplantation and hole application with organic fertilizer can improve its colonization in rhizosphere by 10 times in comparison with its single use in substrate. And its presence resulted in the decline of Foc number over 10 times and 2 weeks delay of the disease development and reduction of disease severity in comparison with the non-B006 treatment. Greenhouse test showed that the addition of B006 powder in substrate can control cucumber wilt effectively with the control efficacy of 53.6%, combined use of 30 mL B006 suspension by drenching at the concentration of 106 CFU g-1 with hole application of 10 g organic fertilizer per seedling during transplantation resulted in the best control performance of 72.2%, while single use of organic fertilizer only resulted in the control performance of 29.4% in comparison with blank control. This study improves our understanding for the interaction of B006 and Foc in cucumber rhizosphere further and provides us a suitable application way of B. subtilis as a bioagent for cucumber wilt management.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Biological Control of Root-rot on Mungbean Plants Incited By Macrophomina phaseolina Through Microbial Antagonists
  •    Suppression of Rhizoctonia solani Root Rot Disease of Clusterbean (Cyamopsis tetragonoloba) and Plant Growth Promotion by Rhizosphere Bacteria
  •    Soil Water Pressure Affects Population Dynamics of Biocontrol Agents of Verticillium dahliae, the Cause of Potato Early Dying
How to cite this article:

Qi-Yao Yang, Ke Jia, Wen-Yi Geng, Rong-Jun Guo and Shi-Dong Li, 2014. Management of Cucumber Wilt Disease by Bacillus subtilis B006 Through Suppression of Fusarium oxysporum f. sp. cucumerinum in Rhizosphere. Plant Pathology Journal, 13: 160-166.

DOI: 10.3923/ppj.2014.160.166

URL: https://scialert.net/abstract/?doi=ppj.2014.160.166

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 

       

       

Curve Bottom