Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Plant Pathology Journal
  Year: 2014 | Volume: 13 | Issue: 2 | Page No.: 133-138
DOI: 10.3923/ppj.2014.133.138
Tri4 and tri5 Gene Expression Analysis in Fusarium graminearum and F. culmorum Isolates by qPCR
Emre Yoruk and Gulruh Albayrak

Abstract:
The tri5 gene cluster is associated with production of trichothecene B type mycotoxins in Fusarium graminearum and F. culmorum. Multifunctional oxygenase and trichodiene synthase encoded by tri4 and tri5 genes, respectively, belonging to this cluster are responsible for production of trichothecenes. In this study, tri4 and tri5 gene expression levels were determined in 14 F. graminearum and 24 F. culmorum isolates causing head blight in wheat, maize and barley collected from different regions of Turkey. Isolates producing oxygenase and trichodiene synthase in high levels were distinguished from low-producers using two-step reverse-transcription comparative quantitative real-time PCR. Slope values were -3.63, -3.4 and -3.4 for amplification of tri4, tri5 and β-tubulin genes, respectively. Cp values were ranged from 17.08±0.0 to 38.82±1.66. The highest tri4 and tri5 expression levels were determined in 4F isolate of F. graminearum. ΔΔCT ratios were calculated as 0.1371 for tri4 gene and 0.865 for tri5 gene. However, maximum tri4 and tri5 expressions were detected in 20F (ΔΔCT = 0.2196) and 9F (ΔΔCT = 0.3761) isolates in F. culmorum, respectively. Quantification of definite toxin content can contribute to determination of pathogen infection grade and to gaining the information about fungal biomass on field crops and their products.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    RAPD-PCR Analysis of Genetic Variation among Isolates of Fusarium graminearum and Fusarium culmorum from Wheat in Adana Turkey
  •    Aggressiveness of Certain Fusarium graminearum Isolates on Wheat Seedlings and Relation with their Trichothecene Production
  •    Pathogenicity of Turkish Crown and Head Scab Isolates on Stem Bases on Winter Wheat under Greenhouse Conditions
How to cite this article:

Emre Yoruk and Gulruh Albayrak, 2014. Tri4 and tri5 Gene Expression Analysis in Fusarium graminearum and F. culmorum Isolates by qPCR. Plant Pathology Journal, 13: 133-138.

DOI: 10.3923/ppj.2014.133.138

URL: https://scialert.net/abstract/?doi=ppj.2014.133.138

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 

       

       

Curve Bottom