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Pakistan Journal of Biological Sciences
  Year: 2018 | Volume: 21 | Issue: 6 | Page No.: 300-306
DOI: 10.3923/pjbs.2018.300.306
 
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Anticariogenic Activities of Derris reticulata Ethanolic Stem Extract Against Streptococcus mutans

Pawitra Pulbutr , Sakulrat Rattanakiat, Natnicha Phetsaardeiam, Patcharapa Modtaku, Rattazart Denchai, Achida Jaruchotikamol and Wanwisa Khunawattanakul

Abstract:
Background and Objective: Streptococcus mutans is a dominant causative pathogen of dental caries, which is a major oral health problem affecting million people worldwide. Derris reticulata is a medicinal plant possessing antimicrobial activity against several Gram-positive pathogenic bacteria. None the less, its effects on growth and cariogenic properties of S. mutans has not been clearly established. This study aimed to investigate the antibacterial and anti cariogenic activities of the D. reticulata ethanolic stem extract. Materials and Methods: The TLC analysis was performed to authenticate the D. reticulata sample. Minimum inhibition concentration and minimum bactericidal concentration were determined by using broth dilution and drop plate methods, respectively. Sucrose dependent and sucrose independent-adherences, biofilm formation and glycolytic pH drop assays were performed to evaluate the anticariogenic activity. Results: The ethanolic stem extract of D. reticulata possessed the antibacterial activity against S. mutans with the MIC and MBC of 0.875±0.250 and 1.750±0.500 mg mL–1, respectively. The extract at the lower concentrations of sub-MIC also had significant inhibitory actions against the cariogenic properties of S. mutans, including surface adherence, biofilm formation and glycolytic acid production. Conclusion: The D. reticulata stem extract had a substantial anticariogenic activities and thus potentially be developed as an oral health care product for dental caries prevention in the near future.
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How to cite this article:

Pawitra Pulbutr, Sakulrat Rattanakiat, Natnicha Phetsaardeiam, Patcharapa Modtaku, Rattazart Denchai, Achida Jaruchotikamol and Wanwisa Khunawattanakul, 2018. Anticariogenic Activities of Derris reticulata Ethanolic Stem Extract Against Streptococcus mutans. Pakistan Journal of Biological Sciences, 21: 300-306.

DOI: 10.3923/pjbs.2018.300.306

URL: https://scialert.net/abstract/?doi=pjbs.2018.300.306

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