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Pakistan Journal of Biological Sciences
  Year: 2014 | Volume: 17 | Issue: 8 | Page No.: 999-1006
DOI: 10.3923/pjbs.2014.999.1006
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Isolation of Hyperoside and Isoquercitrin from Camellia sasanqua as Antioxidant Agents

Agus Sukito and Sanro Tachibana

Two antioxidant active compounds were isolated from the methanol extract of Camellia sasanqua using various in vitro assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching and reducing power assays. The ethyl acetate (EtOAc) fraction of the methanol extract had the highest DPPH radical-scavenging activity with an Inhibition Concentration (IC50) value of 18.3±1.63 μg mL-1. Sephadex LH-20 column chromatography was used to separate the EtOAc fraction into eight fractions (F1-F8). Antioxidant activity was significantly higher in fraction 5 with an IC50 value 14.61±0.02 μg mL-1. Fraction 5 was further separated by HPLC preparative with Capcellpak C18 MG followed by the Cosmosil 5C18-AR-II column, using a guided DPPH radical-scavenging assay. The compounds isolated were identified as: Hyperoside (1) and isoquercitrin (2) after recrystallization from ethanol, based on Mass Spectrum (MS) and Nuclear Magnetic Resonance (NMR) analyses. Their DPPH radical-scavenging activities based on the 50% scavenging concentration decreased in the following order: Isoquercitrin (21.6 mM)>hyperoside (27.5 mM). The antioxidant activities of hyperoside and isoquercitrin were 67.52±0.64 and 64.33±0.51%, respectively, in the β-carotene bleaching assay. These compounds were found to have good reducing powers (OD value: 2.5-3.8) at concentrations of 50-140 μg mL-1, using the potassium ferricyanide reduction method. Although, these compounds are well-known, hyperoside (1) was isolated from this herb for the first time.
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How to cite this article:

Agus Sukito and Sanro Tachibana, 2014. Isolation of Hyperoside and Isoquercitrin from Camellia sasanqua as Antioxidant Agents. Pakistan Journal of Biological Sciences, 17: 999-1006.

DOI: 10.3923/pjbs.2014.999.1006






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