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Pakistan Journal of Biological Sciences

Year: 2013 | Volume: 16 | Issue: 5 | Page No.: 219-225
Research Article

Recovery and Purification of Rapamycin from the Culture Broth of Mtcc 5681

Polavarapu Baby Rani
Department of Biotechnology, Natco Research Center, Hyderabad-500018, India
Battula Suneel Kumar
Department of Biotechnology, Natco Research Center, Hyderabad-500018, India
A.K.S. Bhujanga Rao
Department of Biotechnology, Natco Research Center, Hyderabad-500018, India
S. Sreenivasrao
Department of Biotechnology, Natco Research Center, Hyderabad-500018, India
Mangamoori Lakshmi Narasu
Department of Biotechnology, Natco Research Center, Hyderabad-500018, India
In this study of the recovery and purification of rapamycin from the culture broth of an actinomycetes strain MTCC 5681, we investigated various factors such as biomass separation, suitable solvents for extraction, normal phase and flash chromatographic conditions and selective precipitation to obtain rapamycin in substantially pure form of the product. Adsorption chromatography particularly with normal phase and flash chromatography, in combination with centrifugal decantation is found to be the most suitable for separation as well as purification of rapamycin. Centrifugal decantation technique is likely to emerge as an efficient, industrially scalable, high yielding and economical process for biomass separation. The purity of rapamycin obtained through the method described was 99.4% which has not been reported so far.
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