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Comparison of PCR, Wright Agglutination Test and Blood Culture for Diagnosis of Brucellosis in Suspected Patients |
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Seyedhosssein Hekmatimoghaddam,
Maryam Sadeh,
Mohammad Bagher Khalili,
Mansour Mollaabedin and Alireza Sazmand |
Abstract:
Brucellosis has long been prevalent in Iran, with considerable
medical and economic importance. Timely diagnosis is needed for early management
and effective prevention of its consequences in human beings and animals. Current
diagnostic methods impose peculiar challenges in terms of analytical method
performance. This study compares diagnostic sensitivity, specificity, predictive
Value of Positive (PVP) and Predictive Value of Negative (PVN) for Polymerase
Chain Reaction (PCR), Wright agglutination test and blood culture used for patients
suspected of brucellosis. In 120 patients clinically suspected of brucellosis
and referred by physicians to the Yazd central Medical Laboratory, some relevant
demographic, occupational, nutritional and clinical data were collected. Also,
venous blood samples were drawn for diagnosis of brucellosis using PCR, Wright
agglutination test and blood culture techniques. The most frequent symptom of
patients was arthralgia (82 cases, 68.3%). PCR was positive in 25 cases (20.8%),
wright test in 21 patients (17.5%) and blood culture in 6 cases (5%). In 20
out of 21 wright-positive cases, PCR was positive and all of the culture-positive
patients had positive PCR. Sensitivity, specificity, PVP and PVN of blood culture
compared to PCR (as the gold standard test) were 24, 100, 100 and 86%, respectively,
but the above parameters when PCR is compared with blood culture (as gold standard)
were 100, 83, 24 and 95%, respectively. PCR has better analytical performances
than blood culture for diagnosis of brucellosis and is suitable for confirmation
of Wright-positive cases.
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How to cite this article:
Seyedhosssein Hekmatimoghaddam, Maryam Sadeh, Mohammad Bagher Khalili, Mansour Mollaabedin and Alireza Sazmand, 2013. Comparison of PCR, Wright Agglutination Test and Blood Culture for Diagnosis of Brucellosis in Suspected Patients. Pakistan Journal of Biological Sciences, 16: 1589-1592.
DOI: 10.3923/pjbs.2013.1589.1592
URL: https://scialert.net/abstract/?doi=pjbs.2013.1589.1592
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