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Pakistan Journal of Biological Sciences
  Year: 2013 | Volume: 16 | Issue: 19 | Page No.: 1004-1009
DOI: 10.3923/pjbs.2013.1004.1009
 
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Frequency Distribution and Assessment of Genetic Diversity of Novel Endophyte Alternaria alternata Accessions Isolated from Pongamia pinnata L.

Kartikeya Tiwari

Abstract:
The present study discusses the frequency distribution and genetic diversity of novel fungal endopyte Alternaria alternata within the Pongammia pinnata plant samples. A total of ten plant samples of Pongammia pinnata, Pierre. (Karanja) were collected from specific locations of Sanganer region of Rajasthan for the isolation of fungal endophytes. Of these, maximum frequency of Alternaria alternata (22.29%) were recorded which are morphologically similar but ecologically variant. Efficacy of randomly amplified polymorphic DNA (RAPD), were assessed in seventeen individuals of the primers was GCC 180 where as 10 bands were generated by GCC 181. The similarity coefficient matrix generated for the primers was subjected to algorithm UPGMA (Unweighted Pair Group Method Analysis) and clusters were generated using NTSYS 2.02 pc program. To stabilize the level of relatedness among the seventeen ecologically variant Alternaria alternata accessions, the dendrogram was constructed, which showed that all the isolates were diversified endophytically with in the plant Pongamia pinnata.
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How to cite this article:

Kartikeya Tiwari , 2013. Frequency Distribution and Assessment of Genetic Diversity of Novel Endophyte Alternaria alternata Accessions Isolated from Pongamia pinnata L.. Pakistan Journal of Biological Sciences, 16: 1004-1009.

DOI: 10.3923/pjbs.2013.1004.1009

URL: https://scialert.net/abstract/?doi=pjbs.2013.1004.1009

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