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Pakistan Journal of Biological Sciences
  Year: 2008 | Volume: 11 | Issue: 4 | Page No.: 672-675
DOI: 10.3923/pjbs.2008.672.675
 
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A Method to Study the Effects of Chemical and Biological Reduction of Molybdate to Molybdenum Blue in Bacteria

Yunus Shukor, Burhanuddin Shamsuddin, Othman Mohamad, Khalid Ithnin, Nor Aripin Shamaan and Mohd. Arif Syed

Abstract:
In this research, we modify a previously developed assay for the quantification molybdenum blue to determine whether inhibitors to molybdate reduction in bacteria inhibits cellular reduction or inhibit the chemical formation of one of the intermediate of molybdenum blue; phosphomolybdate. We manage to prove that inhibition of molybdate reduction by phosphate and arsenate is at the level of phosphomolybdate and not cellular. We also prove that mercury is a physiological inhibitor to molybdate reduction. We suggest the use of this method to assess the effect of inhibitors and activators to molybdate reduction in bacteria.

Key words: Molybdate reduction, E. cloacae strain 48, molybdenum blue, inhibitors

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How to cite this article:

Yunus Shukor, Burhanuddin Shamsuddin, Othman Mohamad, Khalid Ithnin, Nor Aripin Shamaan and Mohd. Arif Syed, 2008. A Method to Study the Effects of Chemical and Biological Reduction of Molybdate to Molybdenum Blue in Bacteria. Pakistan Journal of Biological Sciences, 11: 672-675.

DOI: 10.3923/pjbs.2008.672.675

URL: https://scialert.net/abstract/?doi=pjbs.2008.672.675

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