A field study was conducted in 2006 at Sari Agricultural and Natural Resources University, in order to determine the best time for weed control in soybean promising line, 033. Experiment was arranged in randomized complete block design with 4 replications and two series of treatments. In the first series, weeds were kept in place until crop reached V2 (second trifoliolate), V4 (fourth trifoliolate), V6 (sixth trifoliolate), R1 (beginning bloom, first flower), R3 (beginning pod), R5 (beginning seed) and were then removed and the crop kept weed-free for the rest of the season. In the second series, crops were kept weed-free until the above growth stages after which weeds were allowed to grow in the plots for the rest of the season. Whole season weedy and weed-free plots were included in the experiment for yield comparison. The results showed that among studied traits, grain yield, pod numbers per plant and weed biomass were affected significantly by control and interference treatments. The highest number of pods per plant was obtained from plots which kept weed-free for whole season control. Results showed that weed control should be carried out between V2 (26 day after planting) to R1 (63 day after planting) stages of soybean to provide maximum grain yield. Thus, it is possible to optimize the timing of weed control, which can serve to reduce the costs and side effects of intensive chemical weed control.