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Pakistan Journal of Biological Sciences
  Year: 2008 | Volume: 11 | Issue: 15 | Page No.: 1929-1934
DOI: 10.3923/pjbs.2008.1929.1934
A Study on the Effect of Thiotepa on Mice SpermatogenesisUsing Light and Electronic Microscope
D.M. Nejad, J.S. Rad, L. Roshankar, M. Karimipor, A.A. Ghanbari, A. Aazami and M.R. Valilou

Abstract:
The present study was designed to explore the spermatogenic effect of intraperitoneal administration of thiotepa in mice. It is known the chemotherapy could affect testicular function and result in infertility. There are few reports on the side effect of thiotepa on spermatogenesis when are used as anticancer drug. Therefore, in the present study the effect of thiotepa on spermatogenesis was evaluated by electron microscopy. A total of 20 balb/c mice were used in this study. They were divided into control and thiotepa treated groups. Thiotepa was administrated as 2.5 mg kg-1 for 5 days. The mice were sacrificed after 5 weeks and testicular specimens were removed, fixed in boueins fixative and 2.5% Glutaraldehide then prepared for light and electron microscopic study. Light microscopy showed that the thickness of germinal epithelium was decreased in thiotepa treated group and many seminiferous tubules have lost germinal cells. Electron microscopy revealed that in experimental group several intercellular spaces appeared between spermatogonial and sertoli cells. The basement membrane became irregular and intercellular vacuoles were present in sertoli and spermatogonial cells. Nuclear chromatin was condensed and there were several vacuolated mitochondria. It was indicated that thiotepa affected testicular germinal epithelium by both cytotoxic effect and induction of apoptosis.
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How to cite this article:

D.M. Nejad, J.S. Rad, L. Roshankar, M. Karimipor, A.A. Ghanbari, A. Aazami and M.R. Valilou, 2008. A Study on the Effect of Thiotepa on Mice SpermatogenesisUsing Light and Electronic Microscope. Pakistan Journal of Biological Sciences, 11: 1929-1934.

DOI: 10.3923/pjbs.2008.1929.1934

URL: https://scialert.net/abstract/?doi=pjbs.2008.1929.1934

 
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