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Pakistan Journal of Biological Sciences

Year: 2008 | Volume: 11 | Issue: 13 | Page No.: 1708-1712
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Authors

Abdualdaim Mohammed Mukred

Aidil Abd Hamid

Ainon Hamzah

Wan Mohtar Wan Yusoff

Keywords

The bioremediation of polluted groundwater, wastewater aeration pond and biopond sites was investigated using bacteria isolated from these sites located at the oil refinery Terengganu Malaysia. Out of 62 isolates, only 16 isolates from groundwater (8) and wastewater aeration pond (3) and biopond (5) were chosen based on growth medium containing 1% (v/v) Tapis crude oil. Only four isolates; Acinetobacter faecalis, Staphylococcus sp., Pseudomonas putida and Neisseria elongata showed percentage biodegradation of crude oil more than 50% after 5 days using Mineral Salts Medium (MSM). The effect of physical parameters (temperature, pH and agitation) on growth by all four strains showed a maximum growth in MSM medium with 1% Tapis crude oil at 37°C with pH 7 and agitation of 130 rpm.
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