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Pakistan Journal of Biological Sciences

Year: 2007 | Volume: 10 | Issue: 5 | Page No.: 783-787
Research Article

Comparative Analysis of Mitotic Aberrations Induced by Diethyl Sulphate (DES) and Sodium Azide (SA) in Vicia faba L. (Fabaceae)

Tariq Ahmad Bhat
Department of Botany, Cytogenetics and Mutation Breeding Research Laboratory, Aligarh Muslim University, Aligarh-202 002, India
Monika Sharma
Department of Botany, Cytogenetics and Mutation Breeding Research Laboratory, Aligarh Muslim University, Aligarh-202 002, India
M. Anis
Department of Botany, Cytogenetics and Mutation Breeding Research Laboratory, Aligarh Muslim University, Aligarh-202 002, India
The present investigation provides a comparative account of different concentrations (0.01, 0.02, 0.03, 0.04, 0.05 and 0.06%) of diethylsulphate (DES) and Sodium Azide (SA) on mitotic aberrations, seed germination, seedling survival, plant height and mitotic index in Vicia faba L. variety major. The control plants were normal while as treated ones showed significant alterations. The mutagens caused dose dependent decrease in seed germination, seedling survival, plant height and mitotic index. All the parameters were found negatively affected and were positively correlated with mutagenic concentrations. The cytological study revealed various types of mitotic aberrations, among them the dominant were fragments, stickiness, precocious separation, c-metaphase, ring chromosomes, unequal separation, laggards, bridges, micronuclei, disturbed anaphase etc. Stickiness and fragments were more frequent as compared to other types.
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References

  1. Abderrhman, S.M., 1997. Effects of Peganum harmala on root tips of Allium cepa. Cytobiosis, 90: 171-174.
  2. Abraham, S. and B.N. Rajalakshmi, 1989. Production of mitotic abnormalities by magnesium sulphate in Vicia faba L. Cytologia, 54: 559-563.
    CrossRefDirect Link
  3. Anis, M. and A.A. Wani, 1997. Caffeine induced morpho-cytological variability in Fenngreek, Trigonella foenum-graecum L. Cytologia, 62: 343-349.
  4. Badr, A., 1986. Effect of the S-Triazine herbicide tyrbytryn on mitosis chromosomes and nucleic acids in root tips of Vicia faba L. Cytologia, 51: 571-589.
  5. Banerjee, A., 1992. A time course study of the relative cytotoxic effects of extract of different types of Tobacco on Allium cepa mitosis. Cytologia, 57: 315-320.
    Direct Link
  6. Bond, D.A., D.A. Lawes, G.C. Hawtin, M.C. Saxena and J.S. Stephens, 1985. Faba Bean (Vicia faba L.). In: Grain Legume Crops, Summerfield, R.J. and E.H. Roberts (Eds.). William Collins Sons Co. Ltd., London, UK., pp: 199-265.
    Direct Link
  7. Degrassi, F. and M. Rizzoni, 1982. Micronucleus test in Vicia faba root tips to detect mutagen damage in fresh-water pollution. Mutat. Res./Environ. Mutagen. Related Subjects, 97: 19-33.
    CrossRefDirect Link
  8. Bempong, M.A., 1973. Cytological effects of nogalamycin in Tradescantia paludosa microsporocytes. Mut. Res., 21: 323-326.
    PubMed
  9. Evan, H.J., 1962. Chromosome aberrations induced by ionizing radiation. Int. Rev. Cytol., 13: 221-321.
  10. Gaulden, M.E., 1987. Hypothesis: Some mutagens directly alter specific chromosomal proteins (DNA topoisomerase II and peripheral proteins) to produce chromosome stickiness, which causes chromosome aberrations. Mutagenisis, 2: 357-365.
    CrossRefDirect Link
  11. Jayabalan, N. and G.R. Rao, 1987. Gamma radiation induced cytological abnormalities in Lycopersicon esculentum Mill. var. pusa ruby. Cytologia, 52: 1-4.
    CrossRefDirect Link
  12. Reddy, P.M.P.G.H. and H. Bashamohideen, 1991. Inhibition of Mg+2 and Na(+)-K+ ATPases in selected tissues of fish, Cyprinus carpio under fenvalerate toxicity. Biochem. Int., 23: 715-721.
  13. Savage, J.R.K., 1975. Classification and relationships of induced chromosomal structural changes. J. Med. Genet., 12: 103-122.
  14. Sparrow, A.H. and W.R. Singleton, 1953. The use of radio-cobalt as a source of gamma-rays and some effects of chronic irradiation on growing plants. Am. Nat., 87: 29-48.

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