
	Pakistan Journal of Biological Sciences
	Year: 2007 \| Volume: 10 \| Issue: 22 \| Page No.: 3992-3999 DOI: 10.3923/pjbs.2007.3992.3999

Standardization of Mass Production in Three Isolates of Nucleopolyhedrovirus of Helicoverpa armigera (Hübner)

A. Mehrvar, R.J. Rabindra, K. Veenakumari and G.B. Narabenchi

Abstract: The effect of four major parameters, viz., larval age and weight, inoculation dose, incubation temperature and time of harvesting the larvae, on the production of three isolates of nucleopolyhedrovirus of Helicoverpa armigera (HaNPV), viz., Ooty (OTY), Coimbatore (CMB) and Negamum (NGM) were evaluated. Early 5th instar larvae recorded the maximum yield of virus per larva when inoculated with a dose of 5χ10⁵ POB larva‾¹ and incubated at a temperature of 25°C. Also, highest POB yield was recorded when virosed larvae were harvested as cadavers. However, among the isolates tested in this study, CMB isolate collected from Tamil Nadu, India, showed the highest yield per larva in all of the conditions.

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How to cite this article:

A. Mehrvar, R.J. Rabindra, K. Veenakumari and G.B. Narabenchi, 2007. Standardization of Mass Production in Three Isolates of Nucleopolyhedrovirus of Helicoverpa armigera (Hübner). Pakistan Journal of Biological Sciences, 10: 3992-3999.

DOI: 10.3923/pjbs.2007.3992.3999

URL: https://scialert.net/abstract/?doi=pjbs.2007.3992.3999

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