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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 21 | Page No.: 3927-3930
DOI: 10.3923/pjbs.2007.3927.3930
 
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Effects of Different Concentrations of 2,4-D and BAP on Somatic Embryogenesis Induction in Saffron (Crocus sativus L.)

Sh. Rajabpoor , A.V. Azghandi and A. Saboora

Abstract:
To optimize an in vitro protocol for propagation of saffron through somatic embryogenesis, effects of various concentrations of 2,4-D ( 0, 0.25, 0.5, 1, 2, 4 and 8 mg L-1) in combination with BAP (0, 0.25, 0.5, 1, 2, 4 and 8 mg L-1) were studied. Surface-sterilized corms were cut transversally into equal portions and the upper or lower parts were used separately as explants. All treatments were maintained in the darkness at 24±2 °C. After 70 days, the first globular embryos were observed and the number of embryos on each explant reached to its maximum 3 months after culture. Statistical analysis showed that there were significant differences between treatments regarding the number of embryos induced on each explant. The most effective treatment was 2.0 mg L-1 2,4-D + 1.0 mg L-1 BAP for both types of explant (inducing 6.5±1.3 and 35.95±4.9 embryos on each explant for the upper and lower parts, respectively). The average percentages of explants showing embryogenic response were 33.3 and 93.3% for the upper and the lower part of corm tissue respectively in this treatment. Complementary studies are in progress to optimize maturation and germination stages of these somatic embryos.
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How to cite this article:

Sh. Rajabpoor , A.V. Azghandi and A. Saboora , 2007. Effects of Different Concentrations of 2,4-D and BAP on Somatic Embryogenesis Induction in Saffron (Crocus sativus L.). Pakistan Journal of Biological Sciences, 10: 3927-3930.

DOI: 10.3923/pjbs.2007.3927.3930

URL: https://scialert.net/abstract/?doi=pjbs.2007.3927.3930

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