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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 10 | Page No.: 1580-1587
DOI: 10.3923/pjbs.2007.1580.1587
 
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The Effect of Cardiovascular Drugs on Pro-Inflammatory Cytokine Secretion and Natural Killer Activity of Peripheral Blood Mononuclear Cells of Patients with Chronic Heart Failure in vitro

Abdolkarim Sheikhi, Yahya Jaberi, Abdolreza Esmaeilzadeh, Mohammad Khani, Mehdi Moosaeefard and Mostafa Shafaqatian

Abstract:
Recent studies have shown that patients with heart failure over-express pro-inflammatory cytokines which enhance natural killer (NK) activity and negatively influence contractility and contribute to the remodeling of myocardium. The question is that how cardiovascular drugs influence on the cytokines of Peripheral Blood Mononuclear Cells (PBMCs) in Chronic Heart Failure (CHF). To study the effect of cardiovascular drugs on PBMCs-cytokines and NK activity of CHF patients. PBMCs of CHF patients/normal controls collected by Ficoll-paque density centrifugation. NK activity against K562 target cell was measured with MTT colorimetric assay. PBMCs were cultivated in RPMI/FCS, stimulated with phytohaemaglutinin (PHA). Tumor necrosis factor (TNF)-α interleukin (IL)-6, IL-2 and IL-1β of culture supernatants after 24 h incubation with/without furosemide, captopril and digoxin were measured with sandwitch ELISA. Patients had higher NK activity than controls (56.9% ±1.6 vs 50.9% ±1.2, p<0.05). NK activity of patients who already consumed Captopril/Furosemide didn’t show difference with controls. Captopril (3, 1, 0.3 μg mL-1) and Furosemide (5, 2.5, 1.25 μg mL-1) caused a dose dependent inhibition in TNF-α compared with control (329±23, 427±15, 519±19 and 343±19, 430±14, respectively vs. 562±24 pg mL-1 p<0.05). Furosemide caused a dose dependent decrease in IL-6 (421±31, 534±33 vs. 662±41 pg mL-1 p<0.05). Captopril and Furosemide didn’t show any significant effect on IL-1β/IL-2. Digoxin had no significant effect on PBMCs-cytokines. These data suggest that the immunomodulatory effects of Captopril and Furosemide may contribute to their beneficial and no long term adverse effects on PBMCs.
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How to cite this article:

Abdolkarim Sheikhi, Yahya Jaberi, Abdolreza Esmaeilzadeh, Mohammad Khani, Mehdi Moosaeefard and Mostafa Shafaqatian, 2007. The Effect of Cardiovascular Drugs on Pro-Inflammatory Cytokine Secretion and Natural Killer Activity of Peripheral Blood Mononuclear Cells of Patients with Chronic Heart Failure in vitro. Pakistan Journal of Biological Sciences, 10: 1580-1587.

DOI: 10.3923/pjbs.2007.1580.1587

URL: https://scialert.net/abstract/?doi=pjbs.2007.1580.1587

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