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Pakistan Journal of Biological Sciences
  Year: 2006 | Volume: 9 | Issue: 6 | Page No.: 1128-1131
DOI: 10.3923/pjbs.2006.1128.1131
 
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Cloning and Overexpression of rplL Gene of Brucella abortus in Escherichia coli

Gholamreza Zarrini, Zargham Sepehrizadeh, Mojtaba Tabatabaei Yazdi, Qorbane Behzadiyan Nejad and Zuhair Muhammad Hassan

Abstract:
Brucella abortus ribosomal L7/L12 protein is a 12 kDa protein encoded by rplL. This gene was amplified by PCR from B. abortus S19 and cloned in pET16b expression vector. The construct was transformed in Escherichia coli BL21 (DE3) PlysS. The expression was performed by addition 1 mM IPTG (isopropyl-beta-D-thiogalacto- pyranoside) in the medium with OD = 0.6 and incubation continued for 1, 2, 4 and 8 h at 37°C. The highest production (23 mg L-1) level was achieved after 4 h incubation of induction. The recombinant protein was purified by Ni-NTA column and characterized by SDS-PAGE.
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How to cite this article:

Gholamreza Zarrini, Zargham Sepehrizadeh, Mojtaba Tabatabaei Yazdi, Qorbane Behzadiyan Nejad and Zuhair Muhammad Hassan, 2006. Cloning and Overexpression of rplL Gene of Brucella abortus in Escherichia coli. Pakistan Journal of Biological Sciences, 9: 1128-1131.

DOI: 10.3923/pjbs.2006.1128.1131

URL: https://scialert.net/abstract/?doi=pjbs.2006.1128.1131

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