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Pakistan Journal of Biological Sciences
  Year: 2005 | Volume: 8 | Issue: 7 | Page No.: 1016-1020
DOI: 10.3923/pjbs.2005.1016.1020
Epidemiological Analysis of Clinical Isolates of Staphylococcus aureus in Ile-Ife, Nigeria
M.A. Bisi-Johnson , D.O. Kolawole and A.O. Shittu

The isolation, frequency and antibiotic susceptibility pattern of clinical isolates of Staphylococcus aureus at the Obafemi Awolowo University Teaching Hospitals, Ile-Ife, was studied. Staphylococcal isolates obtained from the various specimens were collected from the Microbiology Laboratory of the Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife and also from anterior nares of apparently healthy medical personnel of the hospital. Bacterial identification was based on standard methods which include, colonial morphology, catalase and gram stain reaction. Confirmatory test was by coagulase and DNAse tests. The standard disk agar diffusion method was done employing commercially prepared antibiotic discs (Abtek, Habdiscs) of ampicillin 10 μg, chloramphenicol 10 μg, cloxacillin 5 μg, gentamicin 10 μg, penicillin 1 i.u., streptomycin 10 μg and tetracycline 10 μg. A total of 97 isolates were obtained from 54 male and 32 female patients and 11 healthy medical personnel. A high proportion of isolates (58.8%) were recovered from the middle-aged group. About one-third (28.9%) of all the isolates were obtained from wound samples, which was mostly recovered in patients less than 20 years. The resistance profile is shown as follows: penicillin (100%), ampicillin (62%), tetracycline (45.4%), cloxacillin (47.4%), chloramphenicol (24.7%), streptomycin (23.7%), gentamicin (22.7%) and erythromycin (11.3%). Thirty isolates (30.9%) were resistant to at least three classes of antibiotics. There was no significant difference in resistance to penicillin and chloramphenicol when present data were compared with an earlier report in Ile-Ife. Low rates of resistance to erythromycin were noted in this study and previous investigations conducted in Lagos and Jos, Nigeria. This survey clearly indicates that careful surveillance of multiple resistant S. aureus is important in infection control and stringent measures against nosocomial infections associated with antibiotic-resistant S. aureus are needed in this health institution. Clinicians should enlighten patients on the consequences of indiscriminate use of penicillin and other anti-microbial agents.
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How to cite this article:

M.A. Bisi-Johnson , D.O. Kolawole and A.O. Shittu , 2005. Epidemiological Analysis of Clinical Isolates of Staphylococcus aureus in Ile-Ife, Nigeria. Pakistan Journal of Biological Sciences, 8: 1016-1020.

DOI: 10.3923/pjbs.2005.1016.1020






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