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Pakistan Journal of Biological Sciences
  Year: 2004 | Volume: 7 | Issue: 2 | Page No.: 235-237
DOI: 10.3923/pjbs.2004.235.237
 
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Effects of Genotype on Induction of Callus and Plant Regeneration Potential in vitro Anther Culture of Rice (Oryza sativa L.)
Sharmin Shahnewaz, M.A. Bari , N.A. Siddique and M.H. Rahman

Abstract:
The present studies were carried out to evaluate the response of anthers of six rice genotypes to callus induction and plant regeneration. For callus induction Z2 media supplemented with 2, 4-D 0.5 mg l-1, NAA 2.5 mg l-1 and Kinetin 0.5 mg l- was used. Of the six genotypes, five gave good callusing response. Highest callus induction was observed in BRRI Dhan-29 (8.06%) and lowest in BR-10 (1.42%). Callus induction frequency varied from 1.42-8.06% depending on genotypes. Modified MS medium supplemented with Kinetin 0.5 mg l-1, BAP 2 mg l-1 and NAA 1.0 mg l-1 was used for plant regeneration. The results showed a significant genotypic difference in callus induction and plant regeneration. The regeneration of plantlet from anther derived calli ranged from 57-75% conclusion.
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How to cite this article:

Sharmin Shahnewaz, M.A. Bari , N.A. Siddique and M.H. Rahman , 2004. Effects of Genotype on Induction of Callus and Plant Regeneration Potential in vitro Anther Culture of Rice (Oryza sativa L.). Pakistan Journal of Biological Sciences, 7: 235-237.

DOI: 10.3923/pjbs.2004.235.237

URL: https://scialert.net/abstract/?doi=pjbs.2004.235.237

 
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