
	Pakistan Journal of Biological Sciences
	Year: 2001 \| Volume: 4 \| Issue: 3 \| Page No.: 271-276 DOI: 10.3923/pjbs.2001.271.276

Characterization and Antifungal Evaluation of Chitinase and Laminarinases from Sugar Beet Leaves

Jwanny E. W., S. T. El-Sayed, A. M. Salem and A. N. Shehata

Abstract: Chitinase, laminarinase A and laminarinase B were extracted and purified from Egyptian sugar beet (Beta vulgaris) leaves. Chitinase showed higher heat stability than laminarinase A and laminarinase B when heated at 50°C for 60 min. Chitinase activity was quite stable in water than in buffer, while laminarinase A and laminarinase B activities increased when incubated with 0.1M citrate-phosphate buffer at pH 6.0 to 7.0 for 30 min. The K_m values of chitinase, laminarinase A and laminarinase B were 0.2, 0.27 and 0.074% at pH`s 4.5, 4.5 and 6.5 using colloidal chitin, laminarin A and laminarin B as substrates respectively. Chitinase enzyme was activated by 0.75 mM - mercaptoethanol by 1.6 times, while laminarinase A enzyme was activated by 1.0 mM of CuCl₂, FeSO₄ and EDTA with 2.35, 1.38 and 1.57 times respectively. Laminarinase B enzyme was activated by 1.0 mM Zn SO₄ and K₂SO₄ with 1.3 and 1.18 times respectively. Chitinase, laminarinase A and laminarinase B enzymes have an endo-splitting type of activity. They were able to inhibit the growth and to lyse cell walls of Aspergillus oryza and A. flavus either alone or in combination through the degradation of chitin and laminarin.

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•	Chitinase from Leaves of Beta vulgaris and other Higher Plants

How to cite this article:

Jwanny E. W., S. T. El-Sayed, A. M. Salem and A. N. Shehata , 2001. Characterization and Antifungal Evaluation of Chitinase and Laminarinases from Sugar Beet Leaves. Pakistan Journal of Biological Sciences, 4: 271-276.

DOI: 10.3923/pjbs.2001.271.276

URL: https://scialert.net/abstract/?doi=pjbs.2001.271.276

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