• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Pakistan Journal of Biological Sciences
  2. Vol 4 (1), 2001
  3. 103-106
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Pakistan Journal of Biological Sciences

Year: 2001 | Volume: 4 | Issue: 1 | Page No.: 103-106
DOI: 10.3923/pjbs.2001.103.106

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 298

Search


Authors


Abdulaziz A. Al-Khedhairy

Country: Saudi Arabia

Keywords


  • RT-PCR
  • polyubiquitin
  • cadmium
  • Biomphalaria arabica
Research Article

Quantitative RT-PCR Analysis of Polyubiquitin Gene Expression in Biomphalaria arabica after Exposure to Heat Stress and Cadmium

Abdulaziz A. Al-Khedhairy
This study focused on the analysis of polyubiquitin gene expression in Biomphalaria arabica after exposure to heat stress (42°C, 1-h), a treatment known to induce polyubiquitin gene expression from cell stress responsive promoters, and exposure to lethal and sublethal levels of cadmium (1, 2 and 3 mg/L, 336-h). Polyadenylated RNA was extracted and polyubiquitin gene expression was analysed using quantitative reverse transcription-polymerase chain reaction technology (RT-PCR) with primers complement to the 3` and 5` prime regions of ubiquitin repeats (polyubiquitin). Results were expressed in arbitrary units as a ratio of optical density of polyubiquitn/ actin electrophoretic bands. In heat stressed snails, the expression was 0.242 ± 0.012 and 0.785 ± 0.039 for control and heat-treated individuals, respectively. After cadmium exposure, the expression was 0.327 ± 0.013, 0.298 ± 0.012, 0.523 ± 0.021 and 0.612 ± 0,024 at concentrations of 1, 2 and 3 mg/L respectively. This suggests that alterations in polyubiquitin gene expression may be crucial to withstand cadmium toxicity and may be considered as a biomonitor for toxicity.
PDF Fulltext XML References Citation

How to cite this article

Abdulaziz A. Al-Khedhairy, 2001. Quantitative RT-PCR Analysis of Polyubiquitin Gene Expression in Biomphalaria arabica after Exposure to Heat Stress and Cadmium. Pakistan Journal of Biological Sciences, 4: 103-106.

DOI: 10.3923/pjbs.2001.103.106

URL: https://scialert.net/abstract/?doi=pjbs.2001.103.106

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved