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Pakistan Journal of Biological Sciences
  Year: 2001 | Volume: 4 | Issue: 1 | Page No.: 103-106
DOI: 10.3923/pjbs.2001.103.106
 
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Quantitative RT-PCR Analysis of Polyubiquitin Gene Expression in Biomphalaria arabica after Exposure to Heat Stress and Cadmium

Abdulaziz A. Al-Khedhairy

Abstract:
This study focused on the analysis of polyubiquitin gene expression in Biomphalaria arabica after exposure to heat stress (42°C, 1-h), a treatment known to induce polyubiquitin gene expression from cell stress responsive promoters, and exposure to lethal and sublethal levels of cadmium (1, 2 and 3 mg/L, 336-h). Polyadenylated RNA was extracted and polyubiquitin gene expression was analysed using quantitative reverse transcription-polymerase chain reaction technology (RT-PCR) with primers complement to the 3` and 5` prime regions of ubiquitin repeats (polyubiquitin). Results were expressed in arbitrary units as a ratio of optical density of polyubiquitn/ actin electrophoretic bands. In heat stressed snails, the expression was 0.242 ± 0.012 and 0.785 ± 0.039 for control and heat-treated individuals, respectively. After cadmium exposure, the expression was 0.327 ± 0.013, 0.298 ± 0.012, 0.523 ± 0.021 and 0.612 ± 0,024 at concentrations of 1, 2 and 3 mg/L respectively. This suggests that alterations in polyubiquitin gene expression may be crucial to withstand cadmium toxicity and may be considered as a biomonitor for toxicity.
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How to cite this article:

Abdulaziz A. Al-Khedhairy , 2001. Quantitative RT-PCR Analysis of Polyubiquitin Gene Expression in Biomphalaria arabica after Exposure to Heat Stress and Cadmium. Pakistan Journal of Biological Sciences, 4: 103-106.

DOI: 10.3923/pjbs.2001.103.106

URL: https://scialert.net/abstract/?doi=pjbs.2001.103.106

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