Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2000 | Volume: 3 | Issue: 3 | Page No.: 407-410
DOI: 10.3923/pjbs.2000.407.410
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Purification and Characterization of Polyphenoloxidase from Guava Infected with Fruit-rot Disease

M.A. Razzaque, Z.A. Saud, N. Absar, M.R. Karim and F. Hashinaga

Abstract:
Polyphenoloxidase (PPO), an important oxidative enzyme which played a vital role in the brewing of guava after infection with fruit-rot disease was purified from disease-affected guava. The method was accomplished by ion exchange chromatography of crude enzyme extract on DEAE-cellulose followed by CM-cellulose. The purified enzyme was found to be homogeneous as judged by polyacrylamide disc gel electrophoresis. Molecular weight of the enzyme was estimated to be 178 kDa and 180.5 kDa by gel filtration and SDS-PAGE respectively. The enzyme appeared to be a single polypeptide chain as revealed by SDS-PAGE either in presence or in absence of reducing agent. Optimum activity of the enzyme was at 48°C and pH around 8. Activity was destroyed completely in presence of EDTA, a metal chellator. Purified PPO showed an estimated km value of 4.4 mM for catechol as substrate.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

M.A. Razzaque, Z.A. Saud, N. Absar, M.R. Karim and F. Hashinaga, 2000. Purification and Characterization of Polyphenoloxidase from Guava Infected with Fruit-rot Disease. Pakistan Journal of Biological Sciences, 3: 407-410.

DOI: 10.3923/pjbs.2000.407.410

URL: https://scialert.net/abstract/?doi=pjbs.2000.407.410

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom