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Pharmacologia

Year: 2014 | Volume: 5 | Issue: 9 | Page No.: 357-368
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Authors

Ogbonnia Steve

Olayemi Sunday

Mbaka Godwin

Morgan Yetunde

Usman Abdulrahmam

Odusanya Peter

Ota Declan

Keywords

The study examined the microbial purities, acute and chronic toxicities of a poly-herbal formulation A prepared with Ocimum gratissimum, Momordica charantia and Vernonia amygdalina leaves and formulation B prepared with Ocimum gratissimum, Citrus medica and Momordica charantia leaves used in the treatment of diabetes. The microbial purity was evaluated on some bacterial and fungal organisms using appropriate diagnostic media. Toxicity of the polyherbal preparation was evaluated on Swiss albino mice by administering to the animals oral graded doses of the lyophilized drug in the ranges of 1.0 to 20.0 g kg-1 body weight and observed continuously for the first 4 h and hourly for the next 12 h, then 6 for 56 h (72 h, acute toxicity). Also the effects on the biochemical and haematological parameters were evaluated on the two formulations. The formulations were found to contain heavy loads of both pathogenic and non pathogenic microorganisms higher than officially accepted limits with formulation A having more microbial loads than B. The median acute toxicity values (LD50) of the formulations were, respectively determined to be 15.0 and 15.65 g kg-1 body weight (b.wt.). There was dose dependent significant (p≤0.05) decrease in plasma glucose levels in the animals treated with the two doses (250 and 500 mg kg-1 b.wt.) of the formulations/glibenclamide compared to the diabetic control. The photomicrograph of pancreatic tissues treated with formulations A and B showed significant beta cells survivor compared to diabetic control. There was however, significant increase (p≤0.05) in aspartate aminotransferases (AST) and creatinine levels in the group treated with highest dose of formulation A and B. Significant increase (p≤0.05) in alanine aminotransferases (ALT) levels was observed in the groups treated with formulation B while marginal increase occurred in the group treated with formulation A. The formulations also ameliorated dislipidemia.
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How to cite this article

Ogbonnia Steve, Olayemi Sunday, Mbaka Godwin, Morgan Yetunde, Usman Abdulrahmam, Odusanya Peter and Ota Declan, 2014. Evaluation of Microbial Purity and Toxicity Profile of Two Polyherbal Formulations Used in Nigerian Herbal Medicine. Pharmacologia, 5: 357-368.

URL: https://scialert.net/abstract/?doi=pharmacologia.2014.357.368

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