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Journal of Pharmacology and Toxicology

Year: 2023 | Volume: 18 | Issue: 1 | Page No.: 1-9
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Authors

Benjaporn Buranrat

Benjaporn Buranrat

Maleeruk Utsintong

Maleeruk Utsintong

Keywords

Research Article

Zanthoxylum rhetsa (Roxb.) DC Oil Suppresses the Proliferation, Activates Apoptosis, Inhibits Migration of Human Cervical Cancer Cells

Benjaporn Buranrat Benjaporn Buranrat's LiveDNA and Maleeruk Utsintong Maleeruk Utsintong's LiveDNA
Background and Objective: Zanthoxylum rhetsa (Roxb.) DC are spices for Thai food and it has been used for the treatment of several diseases. However, the activity of Z. rhetsa oil against cancer has not been fully investigated. In this study, the constituents of Z. rhetsa oil were characterized and explored the cytotoxic effects in cervical cancer cells. Materials and Methods: Zanthoxylum rhetsa oil was attained by hydro-distillation and was considered the active compound by Gas Chromatography-Mass Spectrometry (GCMS). The oil was evaluated for antioxidant, cytotoxicity, colony formation, apoptosis and migration in the human cervical cancer HeLa cell line. Results: Twenty-eight known compounds were identified in oil, including monoterpenes, sesquiterpenes and hydrocarbon. The oil inhibited the HeLa cell proliferation with a low IC50 value and decreased colony formation. Significantly, the induction of early apoptotic cell death was induced by the oil and a higher percentage was indicated at 250 μg mL–1. The cell number was reduced after incubating with the oil for 24 hrs and morphological change was observed at 250 μg mL–1. The mechanism of cell death and apoptosis was detected in the induction of ROS formation and were significant at the dose of 100 and 250 μg mL–1. Finally, the oil stimulated anti-migratory activities of the cervical HeLa. Conclusion: Zanthoxylum rhetsa oil was demonstrated to induce cancer cell death, activate early apoptosis and suppress the migration ability of cervical cancer cells through stimulating ROS formation.
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