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Journal of Medical Sciences
  Year: 2019 | Volume: 19 | Issue: 1 | Page No.: 30-37
DOI: 10.3923/jms.2019.30.37
Application of Latex Agglutination and Sandwich ELISA in Detection of Human Hydatidosis
Mousa Abdel Gawad Mousa Ismail, Ibrahim Rabie Aly, Enas Yahia Abu-Sarea, Samah Sayed Abdel Gawad and Raafat Mohamed Shaapan

Abstract:
Background and Objective: Immunodiagnosis has been found to be useful not only in primary diagnosis of cystic echinococcosis (CE) but also for the follow up of patients after treatment. The present study aimed to prepare and purify paramyosin antigen of E. granulosus and evaluate its diagnostic efficacy for detection of echinococcosis by latex agglutination test (LAT) and sandwich Enzyme Linked Immuno-sorbent assay (ELISA) in human serum samples. Materials and Methods: The LAT and sandwich ELISA was carried out using purified hydatid cyst paramyosin antigen by gel filtration. The tested sera included sera from patients infected with hydatidosis (n = 41), sera of patients with other parasites; 8 with Schistosoma mansoni, 5 with Schistosoma haematobium, 10 with fascioliasis, 10 with amoebiasis and 20 tested negative control. Results: The results showed that by using LAT; 10 out of 41 E. granulosus infected samples showed false negative results and the sensitivity of the assay was 75.6%. All 14 out of 33 of other parasites groups were at the border line of the cut off value giving 73.6% specificity. The 5 false positive cases in the group of patients infected with Fasciola and 5 false positive cases in patients with liver amoebiasis. The 3 false positive cases found in patients with Schistosoma mansoni and only one false positive case in patients with Schistosoma haematobium. By using sandwich ELISA, 37 of E. granulosus infected patients had high positive antigen concentration leading to sensitivity of 80.4% while the specificity was 71.7%. Conclusion: The LAT is a suitable and applicable diagnostic method especially when followed by Sandwich ELISA as a confirmatory test for diagnosis of cystic echinococcosis.
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How to cite this article:

Mousa Abdel Gawad Mousa Ismail, Ibrahim Rabie Aly, Enas Yahia Abu-Sarea, Samah Sayed Abdel Gawad and Raafat Mohamed Shaapan, 2019. Application of Latex Agglutination and Sandwich ELISA in Detection of Human Hydatidosis. Journal of Medical Sciences, 19: 30-37.

DOI: 10.3923/jms.2019.30.37

URL: https://scialert.net/abstract/?doi=jms.2019.30.37

 
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