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Research Journal of Microbiology
  Year: 2017 | Volume: 12 | Issue: 4 | Page No.: 266-273
 
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Molecular Identification of MBL Genes blaIMP-1 and blaVIM-1 in Escherichia coli Strains Isolated from Abattoir by Multiplex PCR Technique

Ejikeugwu Chika , Esimone Charles, Iroha Ifeanyichukwu, Igwe David Okeh, Ugwu Malachy, Ezeador Chika, Duru Carissa and Adikwu Michael

Abstract:
Background and Objective: Globally, infections caused by antibiotic resistant bacteria still pose a threat to public health. The widespread use of antibiotics in food-animal production allows resistant strains of microbes to evolve. The contamination of the environment with animal wastes (containing resistant bacteria) is a major route via which human populations become infected by these microbes. Metallo-beta-lactamase (MBL) is one of the resistance mechanism at the disposal of Gram-negative bacteria including Escherichia coli that is chiefly responsible for bacteria resistance to the carbapenems. This study evaluated the antibiogram and occurrence of MBL genes from E. coli isolates recovered from abattoir. Materials and Methods: A total of 120 rectal swab samples from cows in a local abattoir were used for this study. Each of the rectal swab samples were bacteriologically analyzed for the presence of MBL-producing E. coli using the inhibition based assay and multiplex PCR technique. Specific primers for blaIMP-1 and blaVIM-1 MBL genes were used for the multiplex PCR analysis. The data were analyzed using SPSS with chi-square test and one-way analysis of variance. Results: A total of 48 (40%) isolates of E. coli was recovered from the 120 rectal swab samples. The E. coli isolates were highly resistant to ceftriaxone (72.9%), cefoxitin (75%), ceftazidime (100%), ertapenem (83.3%), oxacillin (81.3%), ciprofloxacin (70.8%), cefotaxime (93.8%), aztreonam (97.9%) and nitrofurantoin (75%). Of the 48 E. coli isolates from abattoir, 15 (31%) E. coli isolates were phenotypically confirmed to produce MBLs. However, only 8 E. coli isolates were genotypically confirmed to harbour blaIMP-1 gene by the multiplex PCR used in this study. None of the E. coli phenotypes harboured the blaVIM-1 MBL gene. Conclusion: This study reported the first multiplex PCR detection of blaIMP-1 MBL gene in E. coli isolates from rectal swab of cows in Abakaliki, Nigeria. The molecular identification of the genes encoding MBL production in Gram-negative bacteria from community samples is vital for a reliable epidemiological investigation, surveillance and the forestalling of the emergence and spread of these organisms through the food chain and food-producing animals.
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How to cite this article:

Ejikeugwu Chika, Esimone Charles, Iroha Ifeanyichukwu, Igwe David Okeh, Ugwu Malachy, Ezeador Chika, Duru Carissa and Adikwu Michael, 2017. Molecular Identification of MBL Genes blaIMP-1 and blaVIM-1 in Escherichia coli Strains Isolated from Abattoir by Multiplex PCR Technique. Research Journal of Microbiology, 12: 266-273.

URL: https://scialert.net/abstract/?doi=jm.2017.266.273

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